Sri Madireddy

  1. Home
  2. Tennessee
  3. Tennessee Tech University
  4. Sri Madireddy
Sri Madireddy

Sri B. Madireddy

  • Courses3
  • Reviews4
Rate This Professor

Social Profile:

Join to see

Biography

Tennessee Tech University - Chemistry

Chief Scientist
Hospital & Health Care
Sri Bharat
Madireddy, Ph.D.
West Palm Beach, Florida Area
CHROMATOGRAPHY-MASS SPECTROMETRY SKILLS
• Over 10+ years of hands-on experience in method development, analysis, data interpretation & basic troubleshooting of;
o Waters Xevo TQD coupled with Acquity I-Class UPLC
o Waters-Micromass oa-QTOF coupled with Acquity UPLC (ESI and nano-ESI modes)
o ABSCIEX API 3200 & 3200 QTRAP coupled with Shimadzu Prominence UFLC.
o Varian 320MS QqQ coupled with Prostar HPLC (ESI and APCI modes)
o Varian 1200L QqQ coupled with Prostar HPLC (ESI and APCI)
o Varian CP3800 GC coupled with Saturn 2200 MSn (Liquid Injection, Headspace and SPME)

METHOD DEVELOPMENT AND ANALYSIS SKILLS
• Experience in handling and method development for a variety of analytes (volatile, semi-volatile & non-volatile) in complex matrices;
o Drugs of Abuse: Amphetamine-type Stimulants, Recreational Drugs & Bath Salts.
o Pharmaceuticals, Pesticides, Endocrine-active Compounds & Personal Care Products.
o Microbial Cell Cultures; Proteins, Peptides, Amino Acids, Carbohydrates & Metabolites.
o Wastewater, Surface Water & Activated-sludge.
o Petroleum Products & Residual Accelerants.
o Organic Liquid Explosives.

PROTEOMICS & METABOLOMICS or BIOMARKERS DISCOVERY SKILLS
• Protein extraction by Phenol-Ammonium Acetate-Methanol & TCA-Acetone precipitation.
• Separation of protein by IEF and SDS-PAGE.
• Perform "Bottom-up" proteomic experiments.
• Peptide analysis using DDA and MSe on Waters® nano-ESI-QTOF.
• Protein identification using Waters® ProteinLynx Global Server and MASCOT Servers.
• MCF derivatization of metabolites for GC-MS analysis.
• Structure elucidation of Unknowns/Biomarkers using accurate mass measurements on QTOF.
• Perform SRM and MRM for Unknowns/Biomarker confirmation using QqQ-MS.
• Perform metabolite analysis using MSe and Waters® MarkerLynx.
• Experience with trace compounds extraction protocols; SPE, SPME, Polar Organic Chemical Integrative Sampler (POCIS) Extraction, Liquid-Liquid Extraction & Headspace Extraction.

Experience

  • Tennessee Technological University

    Adjunct Faculty

    o Conduct undergraduate chemistry lectures for CHEM 1110 and CHEM 1010/1020.
    o Prepare course materials and supervise general chemistry laboratories.
    o Evaluate student progress and provide assistance for at-risk students.

  • Tennessee Technological University

    Graduate Research Teaching Assistant

    o Plan and teach basic concepts of chemistry for undergraduates.
    o Graduate teaching assistant for Forensic Chemistry, Organic Chemistry and General Chemistry.
    o Team supervision of lab sessions, coordination of materials and evaluation of lab reports.

  • LabTrust LLC

    Laboratory Specialist - Method Development Coordinator

    • Develop rapid UPLC-MS/MS confirmation methods for urine and oral fluid analysis of
    o Therapeutic drugs; including anticonvulsants, antidepressants (TCAs), SSRIs, barbiturates, benzodiazepines, natural, semi-synthetic and synthetic opiates and stimulants.
    o Drugs of abuse; including bath salts, amphetamine-type stimulants and synthetic cannabinoids.
    • Prepare and maintain SOPs, train new personnel, maintain instrumentation, prioritize laboratory processes, independently evaluate data, assess performance within the laboratory, and several other essential clinical procedure/protocols.

Education

  • Tennessee Technological University

    Master of Science (M.Sc.)

    Chemistry

  • Tennessee Technological University

    Doctor of Philosophy (Ph.D.)

    Environmental Sciences Chemistry

  • Tennessee Technological University

    Adjunct Faculty


    o Conduct undergraduate chemistry lectures for CHEM 1110 and CHEM 1010/1020. o Prepare course materials and supervise general chemistry laboratories. o Evaluate student progress and provide assistance for at-risk students.

  • Tennessee Technological University

    Graduate Research Teaching Assistant


    o Plan and teach basic concepts of chemistry for undergraduates. o Graduate teaching assistant for Forensic Chemistry, Organic Chemistry and General Chemistry. o Team supervision of lab sessions, coordination of materials and evaluation of lab reports.

  • Osmania University

    Bachelors

    Pharmacy

Publications

  • Presentation: Regional and seasonal profiling of selected pharmaceuticals, stimulatory drugs, pesticides, and their metabolites in local water bodies by SPE-HPLC-QqQ and UPLC-QTOF analysis.

    245th ACS National Meeting & Exposition, New Orleans, LA

  • Presentation: Regional and seasonal profiling of selected pharmaceuticals, stimulatory drugs, pesticides, and their metabolites in local water bodies by SPE-HPLC-QqQ and UPLC-QTOF analysis.

    245th ACS National Meeting & Exposition, New Orleans, LA

  • Poster: Differential proteomic approach to determine sub-lethal effects of gold-nanoparticles on Tetrahymena sp.

    Tennessee Academy of Science Centennial Meeting, Nashville, TN

  • Presentation: Regional and seasonal profiling of selected pharmaceuticals, stimulatory drugs, pesticides, and their metabolites in local water bodies by SPE-HPLC-QqQ and UPLC-QTOF analysis.

    245th ACS National Meeting & Exposition, New Orleans, LA

  • Poster: Differential proteomic approach to determine sub-lethal effects of gold-nanoparticles on Tetrahymena sp.

    Tennessee Academy of Science Centennial Meeting, Nashville, TN

  • Poster: Differential proteomic approach determining effects of thiosemicarbazones on cellular mechanisms of tetrahymena

    8th Annual Student Research Day, Tennessee Technological University, Cookeville, TN

  • Presentation: Regional and seasonal profiling of selected pharmaceuticals, stimulatory drugs, pesticides, and their metabolites in local water bodies by SPE-HPLC-QqQ and UPLC-QTOF analysis.

    245th ACS National Meeting & Exposition, New Orleans, LA

  • Poster: Differential proteomic approach to determine sub-lethal effects of gold-nanoparticles on Tetrahymena sp.

    Tennessee Academy of Science Centennial Meeting, Nashville, TN

  • Poster: Differential proteomic approach determining effects of thiosemicarbazones on cellular mechanisms of tetrahymena

    8th Annual Student Research Day, Tennessee Technological University, Cookeville, TN

  • Wipe sampling of amphetamine-type stimulants and recreational drugs on selected household surfaces with analysis by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry

    Journal of Hazardous Materials

    Highlights • Degree of sorption of eight drugs on eleven countertop surfaces was investigated. • Surface composition, volatility and solvent composition played a role in sorption. • Solvent-dependent migration was a key factor of consideration during remediation. • SPME-assisted volatility studies provided evidence for varying degrees of recovery. • Rapid three minute UPLC-QTOF method was developed to quantify the eight compounds.

  • Presentation: Regional and seasonal profiling of selected pharmaceuticals, stimulatory drugs, pesticides, and their metabolites in local water bodies by SPE-HPLC-QqQ and UPLC-QTOF analysis.

    245th ACS National Meeting & Exposition, New Orleans, LA

  • Poster: Differential proteomic approach to determine sub-lethal effects of gold-nanoparticles on Tetrahymena sp.

    Tennessee Academy of Science Centennial Meeting, Nashville, TN

  • Poster: Differential proteomic approach determining effects of thiosemicarbazones on cellular mechanisms of tetrahymena

    8th Annual Student Research Day, Tennessee Technological University, Cookeville, TN

  • Wipe sampling of amphetamine-type stimulants and recreational drugs on selected household surfaces with analysis by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry

    Journal of Hazardous Materials

    Highlights • Degree of sorption of eight drugs on eleven countertop surfaces was investigated. • Surface composition, volatility and solvent composition played a role in sorption. • Solvent-dependent migration was a key factor of consideration during remediation. • SPME-assisted volatility studies provided evidence for varying degrees of recovery. • Rapid three minute UPLC-QTOF method was developed to quantify the eight compounds.

  • Poster: Development of a LC-MS/MS method using selected markers for establishing a chemical fingerprint for clandestine methamphetamine

    237th ACS National Meeting & Exposition, Salt Lake City, UT

  • Presentation: Regional and seasonal profiling of selected pharmaceuticals, stimulatory drugs, pesticides, and their metabolites in local water bodies by SPE-HPLC-QqQ and UPLC-QTOF analysis.

    245th ACS National Meeting & Exposition, New Orleans, LA

  • Poster: Differential proteomic approach to determine sub-lethal effects of gold-nanoparticles on Tetrahymena sp.

    Tennessee Academy of Science Centennial Meeting, Nashville, TN

  • Poster: Differential proteomic approach determining effects of thiosemicarbazones on cellular mechanisms of tetrahymena

    8th Annual Student Research Day, Tennessee Technological University, Cookeville, TN

  • Wipe sampling of amphetamine-type stimulants and recreational drugs on selected household surfaces with analysis by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry

    Journal of Hazardous Materials

    Highlights • Degree of sorption of eight drugs on eleven countertop surfaces was investigated. • Surface composition, volatility and solvent composition played a role in sorption. • Solvent-dependent migration was a key factor of consideration during remediation. • SPME-assisted volatility studies provided evidence for varying degrees of recovery. • Rapid three minute UPLC-QTOF method was developed to quantify the eight compounds.

  • Poster: Development of a LC-MS/MS method using selected markers for establishing a chemical fingerprint for clandestine methamphetamine

    237th ACS National Meeting & Exposition, Salt Lake City, UT

  • Poster: LC-MS/MS and SPME-GC-MS/MS analysis of seized clandestine drugs to establish a chemical fingerprint database.

    66th SWRM/ 62nd SERMACS, New Orleans, LA

Positions

  • American Chemical Society

    Member

  • American Chemical Society

    Member

  • American Society of Mass Spectrometry

    Member

  • American Chemical Society

    Member

  • American Society of Mass Spectrometry

    Member

  • American Chemical Society

    Member

  • American Society of Mass Spectrometry

    Member

  • American Chemical Society

    Member

  • American Society of Mass Spectrometry

    Member

  • American Chemical Society

    Member

  • American Society of Mass Spectrometry

    Member

  • American Chemical Society

    Member

  • American Society of Mass Spectrometry

    Member

  • American Chemical Society

    Member

  • American Society of Mass Spectrometry

    Member

  • American Chemical Society

    Member

  • American Society of Mass Spectrometry

    Member

CHEM 1010

1(1)

  • Level:
  • Credit hours: 0

CHEM 1020

2(1)

  • Level:
  • Credit hours: 0

CHEM 1110

2.5(2)

  • Level:
  • Credit hours: 0
Load More