Resume

• 660082019

  CNPR Certification

  National Association of Pharmaceutical Sales Reps

  Preparing Future Faculty Scholar

  Iowa State University

• 2008

  Iowa State University

  Investigations on inborn errors of metabolism

  Postdoctoral Researcher

  Houston

  Texas Area

  Baylor College of Medicine

  Responsible for question and answer sections in histology labs; proctoring exams

  grading student reports

  tests and exams and managing grades on blackboard.

  Iowa State University

  Upper Iowa University

  Teaches Human Biology and Nutrition

  Instructor

  Des Moines

  Iowa Area

  Ames

  IA

  Serve as a liaison between parent company

  onsite store and labs at Iowa State University.

  Site Administrator

  Promega

  Doctor of Philosophy (PhD)

  Worked on a dissertation entitled \"“Deorphanization of biogenic amine-responsive G protein-coupled receptors (GPCRs) in various model protozoa and Platyhelminthes” May 2012. With this as a basis

  I have transitioned to the profiling of GPCRs in parasitic flatworms

  in attempt to validate alternative drug targets in these parasites. I have assisted in the teaching of histology at the college of vet med. Maintains Biosafety level 2 labs for various project; both collaborative and individual.

  Biomedical Sciences

  Pharmacology Specialization

  ISUPDA

  Iowa State University

• 2006

  MSc

  Worked on a thesis entitled \"Induction of Hypoxia-inducible Factor (HIF-1alpha) in the insulin-producing beta cell line

  INS-1\". I have also been involved in projects entitled \"experts in team\" which emphasizes elements of diversity and culture

  while working to integrate international students into Norwegian culture.

  Medical Technology

  Norwegian University of Science and Technology (NTNU)

  Molecular medicine (intro)

  Interdisciplinary Team work

  Receptor signaling and trafficking

  Patenting and commercialization

  Design of Experiments

• 2005

  Kumasi Center for Collaborative Research

  Kwame Nkrumah University of Science & Technology

  Kumasi

  Baylor College of Medicine

  Upper Iowa University

  Ghana

  Germany

  Worked on collaborative projects using various molecular techniques. I have been involved in epidemiological studies (on onchocerciasis) involving sample collection in various regions of Ghana.

  Research Assistant

  Kumasi Center for Collaborative Research

  Kwame Nkrumah University of Science & Technology

  Kumasi

  Assistant Professor of Biology

  Wichita

  Kansas Area

  Friends University

  Teach courses in the field of biology and biochemistry.

  Adjunct Faculty

  Des Moines

  Iowa Area

  Des Moines Area Community College

  Ames

  Maintenance of Schistosome life cycle

  receptor profiling and RNA interference of putative drug targets. Development of bio-assays and phenotype screening platforms for target discovery.

  Postdoctoral Fellow

  Iowa State University

• 2004

  KGH

  Gh

  Promega

  Grand View University

  Des Moines Area Community College

  Phlebotomy and clinical tests on patient samples taken.

  KGH

  Gh

  Grand View University

  Teaches Molecular Biology and Genetics

  Adjunct Instructor

  Des Moines

  Iowa Area

  English

  Ewe

  Preparing Future Faculty Scholar

  Iowa State University

  Preparing Future Faculty Fellow

  Iowa State University

  Preparing Future Faculty Associate

  Iowa State University

• 2001

  BSc.

  Worked on a collaborative project entitled \"The prevalence of iron deficiency anaemia among pregnant women (attending antenatal checkup at Komfo Anokye Teaching Hospital) and the effect on the mean birth weight of new born babies\" May 2005.

  Biochemistry

  Kwame Nkrumah' University of Science and Technology

  Kumasi

• American Cancer Society

  Immunohistochemistry

  PCR

  Microbiology

  Molecular Biology

  Physiology

  Cell Culture

  Fluorescence Microscopy

  Biochemistry

  Life Sciences

  Histology

  RT-PCR

  Protein Expression

  Molecular Cloning

  ELISA

  RNAi

  Pharmacology

  Drug Discovery

  Biotechnology

  Western Blotting

  Animal Models

  Ergot Alkaloids (Re)generate New Leads as Antiparasitics

  Praziquantel (PZQ) is a key therapy for treatment of parasitic flatworm infections of humans and livestock

  but the mechanism of action of this drug is unresolved. Resolving PZQ-engaged targets and effectors is important for identifying new druggable pathways that may yield novel antiparasitic agents. Here we use functional

  genetic and pharmacological approaches to reveal that serotonergic signals antagonize PZQ action in vivo. Exogenous 5-hydroxytryptamine (5-HT) rescued PZQ-evoked polarity and mobility defects in free-living planarian flatworms. In contrast

  knockdown of a prevalently expressed planarian 5-HT receptor potentiated or phenocopied PZQ action in different functional assays. Subsequent screening of serotonergic ligands revealed that several ergot alkaloids possessed broad efficacy at modulating regenerative outcomes and the mobility of both free living and parasitic flatworms. Ergot alkaloids that phenocopied PZQ in regenerative assays to cause bipolar regeneration exhibited structural modifications consistent with serotonergic blockade. These data suggest that serotonergic activation blocks PZQ action in vivo

  while serotonergic antagonists phenocopy PZQ action. Importantly these studies identify the ergot alkaloid scaffold as a promising structural framework for designing potent agents targeting parasitic bioaminergic G protein coupled receptors.

  Ergot Alkaloids (Re)generate New Leads as Antiparasitics

  Day TA

  Kimber MJ

  Wheeler NW

  Novel RNAi-mediated approach to G protein- coupled receptor deorphanization: proof of principle and characterization of a planarian 5-HT receptor

  Marchant JS

  Day TA

  Haynes CL

  Gruba SM

  ‘Death and Axes': Unexpected Ca2+ Entry Phenologs Predict New Anti-schistosomal Agents

  Carlson

  S.A.

  Rasmussen

  M.A

  Rasmussen

  S.K

  Anderson

  K.L

  Kimber

  M.J

  Day

  T.A.

  Brewer

  M.T.

  Involvement of a putative intercellular signal-recognizing G protein-coupled receptor in the engulfment of Salmonella by the protozoanTetrahymena.

  Steve A. Carlson

  Tim A. Day

  Matthew T. Brewer

  Eukaryotic Cell published ahead of print 23 August 2013

  doi:10.1128/EC.00150-13

  Evidence for a Bacterial Lipopolysaccharide-recognizing G protein-coupled Receptor in the Bacterial Engulfment by Entamoeba histolytica

  Release of Small RNA-containing Exosome-like Vesicles from the Human Filarial Parasite Brugia malayi.

  Walter H. Hsu

  Chong Wang

  Cody J. Alcott

  Brett A. Sponseller

  David M. Wong

  Journal of the American Veterinary Medical Association

  Vol. 243

  No. 6

  Pages 874-881

  Effects of intravenous administration of polymyxin B in neonatal foals with experimental endotoxemia

  Prince Nii

  Agbedanu

  Friends University