Resume

• 2008

  Ph.D.

  The Role of Oxidative Stress in the Pathophysiology of Ovarian Cancer\n\n

  Physiology with a concentration in Reproductive Science

  Wayne State University School of Medicine

  Responsible Conduct of Research (RCR)

  Wayne State University Graduate School

• 2001

  American Association for Cancer Research

  National Postdoctoral Association

  American Society for Reproductive Medicine

  Michigan Physiological Society

  Bachelors of Science

  Biology

  Wayne State University

• Home

  Saed's Laboratory at WSU

  Saed Laboratory

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  RNA isolation

  Immunohistochemistry

  Tissue Culture

  SDS-PAGE

  Confocal Microscopy

  Biochemistry

  Cell Culture

  Immunoprecipitation

  Subcloning

  Cell Biology

  Molecular Biology

  Fluorescence Microscopy

  Protein Chemistry

  Primer Design

  RT-PCR

  Transfection

  Northern Blotting

  Western Blotting

  cDNA

  qPCR

  Oxidative stress: a key regulator of leiomyoma cell survival.

  Oxidative stress: a key regulator of leiomyoma cell survival.

  Anti-Müllerian Hormone (AMH) May Stall Ovarian Cortex Function Through Modulation of Hormone Receptors Other Than the AMH Receptor.

  Sox2 gene amplification significantly impacts Overall Survival (OS) in Serous Epithelial Ovarian Cancer

  Updates of the role of oxidative stress in the pathogenesis of ovarian cancer.

  The Role of Angiogenesis in the Persistence of Chemoresistance in Epithelial Ovarian Cancer.

  Molecular Basis Supporting the Association of Talcum Powder Use With Increased Risk of Ovarian Cancer

  Shifting anaerobic to aerobic metabolism stimulates apoptosis through modulation of redox balance: potential intervention in the pathogenesis of postoperative adhesions.

  Advances in the pathogenesis of adhesion development: The role of oxidative stress

  Chapter: New Insights into the Pathogenesis of Ovarian Cancer: Oxidative Stress

  Nicotinamide adenine dinucleotide phosphate oxidase is differentially regulated in normal myometrium versus leiomyoma

  Xenotransplantation of pre-pubertal ovarian cortex and prevention of follicle depletion with anti-Müllerian hormone (AMH)

  Adhesion phenotype manifests an altered metabolic profile favoring glycolysis.

  Specific point mutations in key redox enzymes are associated with chemoresistance in epithelial ovarian cancer.

  A Single Nucleotide Polymorphism in Catalase Is Strongly Associated with Ovarian Cancer Survival.

  OBJECTIVE:\nThe objective of this study was to determine the expression

  and effect of targeting CD11b with a monoclonal antibody in ovarian cancer cells.\n\nMETHODS:\nCD11b expression was determined in epithelial ovarian cancer (EOC) cell lines and tissues by immunofluorescence and flow cytometry. Cytotoxicity of the CD11b antibody and synergism with chemothearapeutic drugs were determined by the MTT Cell Proliferation Assay in human macrophages

  normal ovarian epithelial cells

  and in both sensitive and chemoresistant EOC cell lines. Cell migration was assessed with a scratch assay and in vivo effects of the CD11b antibody was assessed with a nude mouse ovarian cancer xenograft model. Data was analyzed with either t-tests or one-way ANOVA.\n\nRESULTS:\nCD11b was unexpectedly expressed in several EOC lines and tissues

  but not normal tissues. Targeting CD11b with its monoclonal antibody resulted in intriguing cytotoxic effects in sensitive and chemoresistant EOC lines

  while surprisingly not affecting normal cells. More importantly

  the cytotoxicity of the CD11b antibody when combined with chemotherapeutic drugs (cisplatin or docetaxel) was significantly synergistic

  in both sensitive and chemoresistant EOC cells. The anti-tumorigenic effect of the CD11b antibody was confirmed in an ovarian cancer nude mouse xenograft model.\n\nCONCLUSION:\nHere we identify CD11b as a novel target

  which selectively induces cytotoxicity in ovarian cancer cells.

  Novel expression of CD11b in epithelial ovarian cancer: Potential therapeutic target.

  Evitar (l-Alanyl-l-Glutamine) Regulates Key Signaling Molecules in the Pathogenesis of Postoperative Tissue Fibrosis

  Dr. Ghassan Saeddetti

  Goserelin fosters bone elongation

  but does not prevent ovarian damage

  in cyclophosphamide-treated pre-pubertal mice

  Anti-Mullerian hormone (AMH) regulates stemness-promoting factors in fresh and previously vitrified-warmed ovarian cortex

  The Creation of a Model for Ex Vivo Development of Postoperative Adhesions.

  Nicotinamide adenine dinucleotide phosphate oxidase expression is differentially regulated to favor a pro-oxidant state that contributes to postoperative adhesion development

  King

  Macomb Community College

  Wayne State University School of Medicine

  DS Biotech

  LLC

  Medpace

  Detroit

  - Laboratory Management.\n- Investigating the role of oxidative stress in the pathogenesis of benign gynecologic disorders

  including postoperative adhesions and fibroids as compared to ovarian cancer. \n- Determining the molecular mechanisms underlying the acquisition of chemoresistance in ovarian cancer.

  Postdoctoral Fellow

  Wayne State University School of Medicine

  Cincinnati

  Ohio

  Clinical Research Associate

  Medpace

  Center Campus

  Current courses taught:\nBIOL1000: An introductory lecture and laboratory course in basic biological principles aimed at an understanding of the life processes common to all living things. The major areas of emphasis include the chemical and cellular basis of life

  reproduction

  growth

  development

  heredity

  evolution

  and ecology.

  Adjunct Faculty

  Macomb Community College

  Greater Detroit Area

  Research Assistant

  Wayne State University School of Medicine

  Detroit

  Laboratory management.\nConduct research using state of the art molecular and cellular biology

  and biochemistry techniques to investigate the role of oxidative stress in the pathogenesis of ovarian cancer.

  Graduate Research Assistant

  Wayne State University School of Medicine

  Detroit

  - Laboratory Director\n- Evaluation of markers of oxidative stress

  inflammation

  metabolism

  cell proliferation and apoptosis in the pathogenesis of various diseases\n- Project Management\n- Budgeting

  Director of Laboratory Operations

  DS Biotech

  LLC