Resume

• 2011

  Doctor of Philosophy (Ph.D.)

  Electrochemical DNA Biosensing

  University of Toronto

  A

  University of Karachi

• 2008

  University of Western Ontario

  Ryerson University

  Southern Illinois University

  University of Toronto at Scarborough

  University of Karachi

  Gwangju Institute of Science & Technology

  London

  Canada

  Chemistry lab demonstrator

  Graduate Teaching Assistant

  University of Western Ontario

  Digital Microfluidics for electrochemical biosensing

  University of Toronto

  PhD Student

  PhD in eletrochemical study of basepair mismatches in DNA films on gold surfaces.

  University of Toronto

  Impedance spectroscopy and scanning electrochemical microscopic study of DNA films on gold

  Graduate Student

  London

  Canada Area

  University of Western Ontario

  Synthesis of Halloysite nanotubes composites

  Graduate Research Assistant

  Buk-gu

  Gwangju

  Korea

  Gwangju Institute of Science & Technology

  Carbondale

  Research intensive faculty position in the dependent of chemistry and biochemistry.

  Assistant Professor

  Southern Illinois University

  Karachi

  Pakistan

  Kinetic study of iron(III) complexes with siderophore ligands.

  Graduate Research Student

  University of Karachi

  Graduate studies

  Chemistry

  The University of Western Ontario

• 2006

  English

  Master of Science (M.S.)

  Materials Science and Engineering

  Gwangju Institute of Science and Technology

• 2003

  University of Karachi

  University of Toronto at Scarborough

  Scarborough

  Canada

  Chemistry lab demonstrator

  Graduate Teaching Assistant

  Teaching undergrad chemistry courses.

  Sessional Lecturer

  Toronto

  Canada Area

  Ryerson University

  Yue Yu

• 818

  62/066

  Kurt Edward Geckeler

  A gold nanoparticle-halloysite nanotube

  on a surface of which a gold nanoparticle is formed

  and a method for forming the same are disclosed. In order to form the gold nanoparticle on a surface of the halloysite nanotube

  a gold salt is added to an agitated suspension solution. By the gold salt

  a gold ion is formed on the surface of the halloysite nanotube. If the reducing agent is added to the halloysite nanotube on which the gold ion is formed

  the gold ion is reduced into the gold nanoparticles. The formed gold nanoparticle has the very small size

  and distributed on the surface of the halloysite nanotube. Accordingly

  without the separate protective agent or the surface reformation

  the gold nanoparticle may be easily formed.

  Gold nanoparticle-halloysite nanotube and method of forming the same

  US20090092836 A1

  Kurt Edward Geckeler

• Analytical Chemistry

  Microfabrication

  Scanning Electron Microscopy

  Digital microfluidics

  Materials Science

  Electrochemistry

  Surface Chemistry

  Impedance Spectroscopy

  Nanotechnology

  Self-assembled Monolayers

  Scanning electrochemical microscopy

  Nanoparticles

  Interactions of Metal Ions with DNA and Some Applications

  Heinz-Bernhard Kraatz

  Interactions of Metal Ions with DNA and Some Applications

  Heinz-Bernhard Kraatz

  Electrochemical identification of artificial oligonucleotides related to bovine species. Potential for identification of species based on mismatches in the mitochondrial cytochrome C1 oxidase gene

  Heinz-Bernhard Kraatz

  The effects of oligonucleotide overhangs on the surface hybridization in DNA films: an impedance study

  Michael

  D. M. Dryden

  Analytical CHemistry

  Integrated Digital Microfluidic Platform for Voltammetric Analysis

  Imaging of toll-like receptor microarrays was achieved using scanning electrochemical microscopy with the successful integration of two ferrocene derivatives in order to enhance the background contrast. This investigation has resulted in the novel fabrication of a tuneable

  multiplex

  broad-spectrum bacterial sensor for the interrogation of conserved microbial stimuli.

  An Unexpected Use of Ferrocene: A Scanning Electrochemical Microscopy Study of A Toll-Like Receptor Array and its Interaction with E. Coli.

  Biosensors-on-Chip: A Tropical Review

  Wax Patterned Microwells for Stem Cell Fate Study

  The fabrication of cost effective paper-based analytical devices by wax printing has recently become popular

  by and large

  using cellulose filter papers. Paper-based devices need higher temperature to form hydrophobic barrier across paper substrate

  rely on large working channels (≥500 μm) for liquid handling

  and exhibit lower efficiency (∼50%) of sample mobility. Such limitations confine applications of wax based fabrication. In this work

  we report printability

  fidelity

  and application of wax micropatterns on a non-cellulosic

  non-fibrous

  and non-porous polyethylene terephthalate based substrate (mPET). Resolution of wax printing on mPET was found to be 120 μm for line and 60 μm for channel micropatterns. The wax micropatterns can sustain heat and retain their structural integrity at melting temperature of wax and above (≥120 °C). In application

  wax microwells were patterned on the new substrate in a high throughput fashion

  which formed a suitable niche for mouse embryonic stem cell (mESC) culture either to maintain self-renewal or direct differentiation. This study will open a new direction in wax printing applications not only as a low-cost but a multipurpose fabrication tool.

  Wax Patterned Microwells for Stem Cell Fate Study

  The first sequence-dependent study of DNA films containing metal-mediated base pairs was performed to investigate the charge transfer resistance (RCT) of metal-modified DNA. The imidazole (Im) deoxyribonucleoside was chosen as a highly AgI-specific ligandoside for the formation of Im–AgI–Im complexes within the duplexes. This new class of site-specifically metal-modified DNA films was characterized by UV

  circular dichroism (CD)

  and X-ray photoelectron spectroscopy (XPS). The electrochemical properties of these systems were investigated by means of electron impedance spectroscopy and scanning electrochemical microscopy. Taken together

  these experiments indicated that the incorporation of AgI ions into the DNA films leads to reduced electron transfer through the DNA films. A simple device was proposed that can be switched reversibly between two distinct states with different charge transfer resistance.

  DNA Films Containing the Artificial Nucleobase Imidazole Mediate Charge Transfer in a Silver(I)-Responsive Way.

  Kihwan Choi

  Lab on a Chip

  A digital microfluidic electrochemical immunoassay

  Aaron R Wheeler

  M Dean Chamberlain

  Alphonsus HC Ng

  Kihwan Choi

  Electrochemiluminescence (ECL) is a sensitive analytical technique with great promise for biological applications

  especially when combined with microfluidics. Here

  we report the first integration of ECL with digital microfluidics (DMF). ECL detectors were fabricated into the ITO-coated top plates of DMF devices

  allowing for the generation of light from electrically excited luminophores in sample droplets. The new system was characterized by making electrochemical and ECL measurements of soluble mixtures of tris(phenanthroline)ruthenium(II) and tripropylamine (TPA) solutions. The system was then validated by application to an oligonucleotide hybridization assay

  using magnetic particles bearing 21-mer

  deoxyribose analogues of the complement to microRNA-143 (miRNA-143). The system detects single nucleotide mismatches with high specificity

  and has a limit of detection of 1.5 femtomoles. The system is capable of detecting miRNA-143 in cancer cell lysates

  allowing for the discrimination between the MCF-7 (less aggressive) and MDA-MB-231 (more aggressive) cell lines. We propose that DMF-ECL represents a valuable new tool in the microfluidics toolbox for a wide variety of applications.

  Electrochemiluminescence on digital microfluidics for microRNA analysis

  Aaron Wheeler

  Electrochemistry

  biosensors and microfluidics are popular research topics that have attracted widespread attention from chemists

  biologists

  physicists

  and engineers. Here

  we introduce the basic concepts and recent histories of electrochemistry

  biosensors

  and microfluidics

  and describe how they are combining to form new application-areas

  including so-called “point-of-care” systems in which measurements traditionally performed in a laboratory are moved into the field. We propose that this review can serve both as a useful starting-point for researchers who are new to these topics

  as well as being a compendium of the current state-of-the art for experts in these evolving areas.

  Electrochemistry

  biosensors and microfluidics: a convergence of fields

  Mohtashim

  Shamsi

  University of Toronto