Resume

• 1995

  Japanese

  Ph.D

  Expertise: Nano and microfluidics

  bio-microstructure hybrid system

  microrobotics

  Electrical Engineering

  Cornell University

• Image Processing

  Higher Education

  Mathematica

  University Teaching

  Experimentation

  Simulations

  Nanotechnology

  Research

  Mathematical Modeling

  Physics

  Microfluidics

  Nanoparticles

  Science

  Theory

  Microscopy

  LaTeX

  Matlab

  Nanofabrication

  Signal Processing

  Numerical Analysis

  Measurement of Protein 53 Diffusion Coefficient in Live HeLa Cells Using Raster Image Correlation Spectroscopy (RICS)

  Mien-Chie Hung

  Chao-Kai Chou

  Pei-Hsiang Tsou

  Hirohito Yamaguchi

  Ying-Nai Wang

  Measurement of Protein 53 Diffusion Coefficient in Live HeLa Cells Using Raster Image Correlation Spectroscopy (RICS)

  Mien-Chie Hung

  Jennifer L. Hsu

  Hong-Jen Lee

  Ying-Nai Wang

  Hirohito Yamaguchi

  Jung-Mao Hsu

  Chun-Te Chen

  Heng-Huan Lee

  [Abstract] Signal transduction is a dynamic process that regulates cellular functions through multiple types of biomolecular interactions

  such as the interactions between proteins and between proteins and nucleic acids. However

  the techniques currently available for identifying protein-protein or protein–nucleic acid complexes typically provide information about the overall population of signaling complexes in a sample instead of information about the individual signaling complexes therein. We developed a technique called “microchannel for multiparameter analysis of proteins in a single complex” (mMAPS) that simultaneously detected individual target proteins either singly or in a multicomponent complex in cell or tissue lysates. We detected the target proteins labeled with fluorophores by flow proteometry

  which provided quantified data in the form of multidimensional fluorescence plots. Using mMAPS

  we quantified individual complexes of epidermal growth factor (EGF) with its receptor EGFR

  EGFR with signal transducer and activator of transcription 3 (STAT3)

  and STAT3 with the acetylase p300 and DNA in lysates from cultured cells with and without treatment with EGF

  as well as in lysates from tumor xenograft tissue. Consistent with the ability of this method to reveal the dynamics of signaling protein interactions

  we observed that cells treated with EGF induced the interaction of EGF with EGFR and the autophosphorylation of EGFR

  but this interaction decreased with longer treatment time. Thus

  we expect that this technique may reveal new aspects of molecular interaction dynamics.

  mMAPS: A Flow-Proteometric Technique to Analyze Protein-Protein Interactions in Individual Signaling Complexes

  Mien-Chie Hung

  Chin B. Su

  Hirohito Yamaguchi

  [Abstract] A simple microfluidic 3D hydrodynamicflow focusing device has been developed and demonstrated quantitative determinations of quantum dot 525 with antibody (QD525-antibody) and hemagglutinin epitope tagged MAX (HA-MAX) protein concentrations. This device had a step depth cross junction structure at a hydrodynamicflow focusing point at which the analyte stream was flowed into a main detection channel and pinched not only horizontally but also vertically by two sheath streams. As a result

  a triangular cross-sectional flow profile of the analyte stream was formed and the laser was focused on the top of the triangular shaped analyte stream. Since the detection volume was smaller than the radius of laser spot

  a photon burst histogram showed Gaussian distribution

  which was necessary for the quantitative analysis of protein concentration. By using this approach

  a linear concentration curve of QD525-antibody down to 10 pM was demonstrated. In addition

  the concentration of HA-MAX protein in HEK293 cell lysate was determined as 0.283 ± 0.015 nM. This approach requires for only 1 min determining protein concentration. As the best of our knowledge

  this is the first time to determinate protein concentration by using single molecule detection techniques.

  Microfluidic three-dimensional hydrodynamic flow focusing for the rapid protein concentration analysis

  Takeyuki Misawa

  Keiyo Nakano

  Hiroshi Miyamoto

  Masayuki Yasuike

  Pei-Hsiang Tsou

  Rapid antibiotic efficacy screening with aluminum oxide nanoporous membrane filter-chip and optical detection system.

  Kameoka

  Texas A&M University

  Cornell University

  Texas A&M University

  Associate Professor

  Texas A&M University

  Post Doctoral Associate、Nanobiotechnology Center

  Cornell University