Resume

• 2017

  Accelerated Bachelor's of Science

  Registered Nursing/Registered Nurse

  Director (ETSU International Choir

  2017 - 2018)

  East Tennessee State University

  Magna Cum Laude

• 2014

  Master’s Degree

  Chemistry

  Graduate and Professional Student Association (GPSA) ETSU

  American Chemical Society

  East Tennessee State University

• 2012

  Master's Degree

  Thesis titled “Growth performance

  bioconcentration of mineral elements and assessment rhizosphere microorganisms of African Yam Beans

  Sphenostylis stenocarpa (Hoechst ex. A. Rich.) Harms grown on dumpsite soils in Benin City ”

  2014. Published in International Journal of Microbiology and Mycology

  1(1): 23 – 30 and Journal of Basic and Applied Sciences

  357–365.\n\nCONFERENCES PAPERS\n1. Assessment of rhizosphere microorganism and mineral element bioconcentration of Sphenostylis stenocarpa Hoshst Ex. A. Rich Harms grown on two dumpsite soils in Benin City.\n\n2. Growth response and yield performance of Sphenostylis stenocarpa Hoshst Ex. A. Rich Harms grown on two dumpsite soils in Benin City. OSAZEE J.O. and Mgbeze

  G. C. \n\nBoth papers presented at the 22nd Annual Conference of the Botanical Society of Nigeria (BOSON) organized by the University of Nigeria

  Nsukka between 26th – 29th May

  Plant Biology and Biotechnology (Environmental Plant Physiology/Phytoremediation Technology Option)

  University of Benin

  PhD Grade (GPA = 4.3/5.0)

• 2006

  Bachelor's Degree

  Research Project titled “Microorganisms contaminating Pepper (Capsicum species) in a local market in Benin City

  Nigeria”

  2008. Published in Nigerian Journal of Life Sciences

  1(2): 92 – 95.\n\n

  Microbiology

  General (GPA=3.5/5.0)

  Class Representative Microbiology

  2007 and 2008

  \nSports Director - Christian Community on Campus (CCC) University of Benin (2007 - 2008).

  University of Benin

  Second Class Upper Division

  Registered Nurse

  Tennessee State Board of Nursing

• 2004.

  Associate's Degree (Diploma)

  Research Project titled \" Microbial Spoilage of Banana and Plantains\"

  Applied Microbiology

  University of Benin

  Upper Credit

• Drug Discovery and Evaluation

  Start-ups

  PowerPoint

  Statistics

  Laboratory

  New Business Development

  Entrepreneurship

  Product Development

  Bioremediation

  Strategic Planning

  Organic Synthesis

  Business Strategy

  DNA Barcoding

  Data Analysis

  Research

  Microbiology

  Microsoft Excel

  Chemistry

  Public Speaking

  Science

  In-vitro screening of antibacterial potentials of Aspilia africana leaves.

  Ogunsan

  F. E

  Daniel

  O. E

  ABSTRACT \n\nIn-vitro screening of antibacterial potentials of aqueous

  ethanolic and methanolic extracts of\nAspilia africana leaves was carried out using agar well diffusion method. Data obtained from\nthis study indicated that the leaf extracts of A. africana possessed antibacterial activity against\nStaphylococcus aureus

  Pseudomonas aeruginosa

  and Klebsiella pneumoniae. The\nmethanolic and ethanolic extract of A. africana showed antibacterial activity with the\ndiameter of zone of inhibition of 9.0 - 16.0 mm and 7.17 - 14.17 mm against S. aureus

  8.0 –\n14.67 mm and 8.83 - 13.17 mm against K. pneumonia and 7.0 - 14.0 mm and 7.50 - 12.33 mm\nagainst P. aeruginosa respectively. Methanolic extract of A. africana was observed to be\nmore potent

  inhibiting all isolates thus showing higher antibacterial activity than the\nethanolic and aqueous extracts. The efficacy of the extracts was further exhibited as the S.\naureus used for the study

  was resistant to the positive control (gentamicin) while been\nsensitive to the extracts. The test organisms were not sensitive to the extractants alone. The\nefficacy of the extracts towards inhibition of the micro organisms increased with increased\nconcentration. The Minimum Inhibitory Concentration and Minimum Bactericidal\nConcentration of the extracts on the test organisms also increased in the following order;\naqueous < ethanolic < methanolic. Findings from this study further showed that plants are\npotential sources of new drugs for treating infections caused by these antibiotic resistant\nclinical pathogens.\n\n\nKey words: Antibacterial activity

  A. africana

  agar well diffusion assay

  methanolic extract.

  In-vitro screening of antibacterial potentials of Aspilia africana leaves.

  Oziengbe

  E. O

  Antifungal activity of copper sulphate against Colletotrichum gloeosporioides.

  Molecular Docking

  Synthesis and Evaluation of Pyrrolo[2

  1-c][1

  4]benzodiazepines Derivatives as Non-β-lactam β-lactamases Inhibitors

  Ikhajiagbe

  B

  Chijioke – Osuji

  C. C.

  Abstract \nThis study investigated the heavy metal contents and microbial diversity of waste engine oil- polluted soil in some public and commercial centres in Benin City metropolis

  Nigeria. The study was carried out in four Local Government Areas (Ikpoba Okha

  Ovia North East

  Egor and Oredo) within Benin Metropolis; the administrative headquarters of Edo State of Nigeria. Certain plots within the study area were selected based on the presence of waste engine oil spill on the ground

  either due to activities of mechanics

  parked vehicles

  stationary diesel engines

  or oil dump sites. However

  in the present study only mechanic workshops (MW)

  bakeries (BK)

  generating plant houses (GN)

  as well as spare part dealer shops (SP) located within the City were selected. Field reconnaissance studies were carried to estimate the extent of pollution on surrounding soil

  using the presence and absence of some flora and fauna

  and developmental defects on some flora. Top soil (0 - 10 cm) was randomly collected from about 30 cm from the periphery of the spill sites and then pooled together to obtain composite samples. These were labelled appropriately

  and immediately transported in polythene bags

  covered with aluminium foil papers

  to the laboratory for heavy metal and microbial assay. Keywords: Hydrocarbons

  heavy metal

  waste engine oil

  bio-remediation.

  Heavy metal contents and microbial diversity of waste engine oil – polluted soil in some public and commercial centres in Benin City metropolis

  Nigeria.

  Victoria Palau

  Crystal Whitted

  Dobrusia Bialonska

  Synthesis and biological activity of fused tetracyclic Pyrrolo [2

  1- c][1

  4]benzodiazepines.

  G. C. Mgbeze

  Survey of dumpsite plant composition

  assessment of rhizosphere microorganisms and growth performance of Sphenostylis stenocarpa grown on two dumpsite (CAPITOL and NITEL ROAD) soils in Benin City was investigated. Control treatment was top soil. A total of 9 and 30 flora were observed at the CAPITOL and NITEL ROAD dumpsites respectively. Analysis of the rhizosphere soils of the plants grown in dumpsite soils at different amendments showed a total heterotrophic bacterial count ranging from 1.57 x 10^4 to 4.18 x 10^4cfu/g and a total heterotrophic fungal count in the various rhizosphere soils ranged from 5.05 x 10^3 to 1.68 x 10^4 cfu/g. The bacterial isolates from the rhizosphere soil samples were Arthrobacter sp.

  Bacillus sp

  Pseudomonas sp.

  Escherichia coli

  Enterobacter sp.

  Klebsiella sp.

  Micrococcus sp. and Staphylococcus sp. The fungal isolates were Aspergillus sp

  Mucor sp

  Fusarium sp

  Penicillium sp

  Trichoderma sp. and Saccharomyces sp. Bacillus sp.

  Pseudomonas sp.

  Penicillium sp. and Aspergillus sp. Aspergillus sp. 100 % (present in all dumpsite soils analyzed) had the highest frequency of occurrence amongst the isolates.Percentage seedling emergence was significantly reduced from 86.67± 13.33 % - 100.00 ± 0.00 % in control (top) soil to 60.00 ± 0.00 % to 93.33 ± 6.67 % in CAPITOL dumpsite soil. Shoot height at 6 weeks after planting (WAP) was significantly (p < 0.05) increased from 78.33± 18.53 cm in the control soil through 131.50 ± 18.79 cm in the CAPITOL dumpsite soil to 186.33 ± 13.68 cm in NITEL road dumpsite soil

  all without amendment. Number of leaves at 6 WAP increased on addition of FYM in both soil types. In all parameters observed

  it was noted that the control treatment did better than the plants grown in the dumpsite soils with increased amendment.

  Assessment of Floristic

  Microbial Composition and Growth of Sphenostylis stenocarpa (Hochst Ex A. Rich) in Soil from Two Dumpsites in Benin City

  Nigeria

  Carla Slebodnick

  The rapid emergence of antimicrobial resistance has threatened the efficacy of first-line antibiotic therapeutics and presents health care systems with serious challenges. There is an urgent need to design and develop both additional therapeutic alternatives and enhanced antibacterial stewardship. Production and dissemination of β-lactamases as the major cause of bacterial resistance to β-lactam antibiotics initiates the catalytic hydrolysis and therefore

  inactivates the β-lactams. A program was initiated to discover a series of novel non-β-lactam inhibitors containing 7-membered ring dilactam scaffolds to explore potent inhibitors of serine β-lactamases. All designed compounds 1-13 passed the Lipinski’s rule of five test which is used to identify potential drug-like compounds and were docked into the active site of TEM-1 β-lactamase using ParDOCK software. Pyrrolo[2

  1-c][1

  4]benzodiazepine (PBD) derivatives 1-7 were synthesized and the association of ligands with purified enzymes in solution was examined. The competitive enzyme inhibition kinetics results have shown that PBDs are quite weak inhibitors when tested against TEM-1 and P99. The limited inhibitory activity of the compounds 1-7 was assumed to happen due to the lack of solubility and bulky nature of the molecules

  thus limiting the optimal ligand-enzyme interactions. Analogs 11-13 were further synthesized to reduce the steric hindrance of the PBDs while promoting the electrophilicity of the active lactam. Compounds 8-13 were also exhibited low inhibitory activity against both enzymes.

  Structure-Based Discovery and Synthesis of Pyrrolo[2

  1-c][1

  4]-benzodiazepine Derivatives as Non-β-lactam Serine β-lactamase Inhibitors

  Adesanmi

  E. A

  Daniel

  O. E

  ABSTRACT \nThe antibacterial efficacy of crude aqueous

  ethanolic and methanolic extracts of Ocimum gratissimum and Ageratum conyzoides L. against Staphylococcus aureus

  Pseudomonas aeruginosa and Escherichia coli weredetermined using disc diffusion method. All the extracts (aqueous

  ethanol

  methanol extracts) showed marked antibacterial activity. Results obtained revealed that methanolic extracts were more potent inhibiting all isolates with zones of inhibition ranging from 7 mm – 17 mm for A. conyzoides L. and 9 mm – 19 mm for O. gratissimum. Ethanolic extracts showed 7 mm – 16 mm for both plants. Aqueous extracts showed the least with diameter zones of inhibition ranging from 7mm – 12 mm for A. conyzoides L. and 6 mm – 10 mm for O. gratissimum. When the antibacterial activity of each of the plant extracts were compared for aqueous

  ethanol and methanol extracts

  there was no observable difference noticed in their spectra of activity. The efficacy of both plants towards inhibition of the micro organisms increased with increased concentration. The antibacterial activity of the extracts could be enhanced if the components were purified. These plants therefore

  are potential sources of new drugs for treating infections caused by these clinical pathogens. \n\nKeywords:\n Disk diffusion method

  antimicrobial activity

  A. conyzoides

  O. gratissimum

  methanolic extract.

  Comparative analysis of Ageratum conyzoides L. and Ocimum gratissimum extracts on some clinical bacterial isolates.

  Daniel

  E. O.

  Obayagbona

  N. O

  Abstract\nSeveral methodologies were utilized to evaluate the microbiological and physico chemical properties of top soil samples bored from four municipal waste dumpsites and a farmland (control sample) all located in Benin City

  Edo State. The soil samples were obtained during the month of January

  2013. The mean aerobic bacterial counts for the soil samples ranged from \n9.7 × 10^3 cfu/g for the control soil to 1.80 × 10^4 cfu/g for the soil sourced from the dump site at Ikheuniro. The mean heterotrophic fungal counts varied from 7.0 × 10^2 cfu/g for capitol dumpsite to 3.3 × 10^3 cfu/g for the control soil. Ten (10) microbial isolates were characterized and identified; Bacillus sp.

  Pseudomonas sp.

  Aeromonas sp.

  Enterobacter sp.

  Klebsiella sp. and Staphylococcus sp.

  Aspergillus sp.

  Mucor sp.

  Saccharomyces sp. and Fusarium sp. respectively. Both Bacillus sp. and Pseudomonas sp. were the most dominant amongst the bacterial isolates whilst Staphylococcus sp. was the least occurring bacterial isolate. Aspergillus sp. was the highest occurring fungal isolate while the least isolated fungal culture was Saccharomyces sp. The physico chemical results showed values which ranged from 5.60 to 8.08

  164.00 μS/cm to 540.00 μS/cm

  2.378 mg/kg to 3.444 mg/kg

  0.009 mg/kg to 0.016 mg/kg for pH

  electrical conductivity

  sulphate and cadmium. Despite the positive impacts of the dumped municipal wastes on the microbial and organic properties of the analyzed soils

  disposal of municipal wastes in open dump sites is an archaic and unsustainable option in the management of municipal wastes.

  Microbiological and Physiochemical Analyses of Top soils obtained from four municipal waste dumpsites in Benin City

  Nigeria.

  Daniel

  E.O.

  Iseh

  E.C.

  Daniel

  E.O.

  OSAZEE

  J.O. And Iseh

  E.C. (2013). Total Microbial count and nutritional analysis of four weaning foods sold in Benin City. NISEB Journal

  13 (3&4): 61 – 64.\n\nABSTRACT: Four (4) different weaning food samples purchased from Oba market located in Benin City

  Edo State

  Nigeria encoded A

  B

  C and Ogi (Nigerian indigenous weaning food) respectively were analyzed for their microbial and nutritional content in this study. Enumeration of total aerobic viable count was done using Nutrient agar. Eosin methylene blue (EMB) agar was used for coliform count

  Salmonella - Shigella agar was used for Salmonella and Shigella count

  mannitol salt agar for staphylococcal count and Sabouraud dextrose agar for mould and yeast counts. Total aerobic counts of organisms in weaning food C (21 ± 1.4 ×103 cfu g -1) was significantly (P< 0.05) higher while weaning B and A had lowest aerobic count of 0.5 ± 0.04 ×103 cfu g -1 and 0.5 ± 0.07 ×103 cfu g -1 respectively. Ogi has a total aerobic count of 1.0 x 104. No Salmonella - Shigella count was observed in all weaning foods sampled. Coliform count and fungi and mould counts were only observed in Ogi and weaning food C. High percentage carbohydrate (73.75 ± 0.61) and moisture (9.3 ± 0.8) was observed in ogi. \nKeywords: Total aerobic counts

  Weaning food

  Microbial analysis

  Nutritional analysis

  coliform. \n

  Total Microbial count and nutritional analysis of four weaning foods sold in Benin City.

  I am passionate about research and developing new products that would make the world a better and safer place to live in. With the increase in antimicrobial resistance of deadly pathogens world over

  increased cases of neurodegenerative diseases and cancers

  there is need for more researches in these area to curb the mortality caused by these diseases and their causative agents. This is what drives my research sense currently.

  Joseph Osamudiamen

  Osazee

  The Evangelical Lutheran Good Samaritan Society

  Johnson City Medical Center

  Johnson City

  East Tennessee State University

  Gracefield Farms

  ETSU Department of Intercollegiate Athletics

  Language & Culture Resource Center

  East Tennessee State University

  Johnson City

  Tuition Scholar

  East Tennessee State University

  Dr Abbas Shilabin Lab

  Department of Chemistry

  ETSU

  Johnson City

  TN

  Molecular Docking

  Synthesis and Evaluation of Pyrrolo[2

  1-C][1

  4]benzodiazepine Derivatives as Novel Non-β-lactam β-Lactamase Inhibitors

  Graduate Research Assistant

  East Tennessee State University

  Benin City

  Nigeria

  Gracefield Farms

  East Tennessee State University

  Johnson City

  Tennessee

  Supervised undergraduate research students\nInstructed in Organic Chemistry lab course\nAssisted professors in teaching Organic Chemistry I and II class and led review sessions for students\nInstructed General Chemistry lab course

  Graduate Teaching Assistant

  Johnson City

  Tennessee Area

  Tutoring student athletes Organic Chemistry

  General Chemistry

  Probability and statistics

  Microbiology

  Biology for majors and non majors and introduction to Chemistry Survey\n

  Tutor

  East Tennessee State University

  ETSU Department of Intercollegiate Athletics

  Johnson City

  Tennessee Area

  Johnson City Medical Center

  Johnson City Medical Center

  Johnson City

  Sioux Falls

  South Dakota Area

  Charge Nurse

  The Evangelical Lutheran Good Samaritan Society

  Graduate Member

  Nigerian Institute of Management (Chartered)

  Graduate Student Member

  American Chemical Society

  Member

  The Nigerian Society for Experimental Biology (NISEB)

  Member

  Nigerian Society of Biochemistry and Molecular Biology (NSBMB)

  Global Outreach Membership

  American Society for Microbiology

  Bini

  English

  First Place Award Winner - Natural Sciences (Group B) - Master Student Poster Presentation

  2015 Appalachian Student Research Forum

  Natural Sciences Session

  D.P. Culp Center

  East Tennessee State University

  Johnson City

  TN 37604

  USA

  April 8 – 9

  2015. Poster Presentation 1st prize winner). Osazee

  J. O. and Shilabin

  A. G.

  Fragment-Based Design and Evaluation of Pyrrolo[2

  1-C][1

  4]benzodiazepine Derivatives as Novel Non-β-lactam β-Lactamase Inhibitors.

  Appalachian Student Research Forum & Office of Research and Sponsored Programs

  ETSU

  Graduate Student Star (2)

  Chair

  Chemistry - ETSU

  ETSU Public Performance Tuition Scholarship

  ETSU Multicultural Center