Resume

• 2015

  Doctor of Philosophy - PhD

  Dissertation: Linking Central Metabolism to Bacterial Pathogenesis: Three Transketolases Sustain Carbon Flux to Promote Salmonella Virulence During Periods of Oxidative and Nitrosative Stresses

  Medical Microbiology and Immunology

  Creighton University

• 2013

  Master's degree

  Dissertation: Transketolases Mediate Sensitivity of Salmonella to Antimicrobial Agents

  Biology

  General

  University of Nebraska at Kearney

  Scientific Writing and Publishing

  Best Practices in Curriculum Design

  Teaching and Assessment

• 2008

  Bachelor's degree

  Biology

  General

  University of Nebraska at Kearney

• Microbiology

  Teamwork

  Polymerase Chain Reaction (PCR)

  Molecular Biology

  Molecular Cloning

  Research

  Mentoring

  Accountability

  Public Speaking

  Decision-Making

  Time Management

  Molecular & Cellular Biology

  Analytical Skills

  Teaching

  Presentation Skills

  Presentations

  Communication

  Data Analysis

  Life Sciences

  Written Communication

  14 Research Poster Presentations at National and Regional Scientific Meetings and Conferences

  14 Research Poster Presentations at National and Regional Scientific Meetings and Conferences

  Salmonella enterica serovar Typhimurium has three transketolase enzymes contributing to the pentose phosphate pathway

  Salmonella enterica serovar Typhimurium has three transketolase enzymes contributing to the pentose phosphate pathway

  The Nucleotide Excision Repair Pathway Protects Borrelia burgdorferi from Nitrosative Stress in Ixodes scapularis Ticks

  Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella

  15+ Oral Research Presentations at National and Regional Scientific Meetings and Conferences

  Magnesium homeostasis protects Salmonella against nitrooxidative stress

  Magnesium homeostasis protects Salmonella against nitrooxidative stress

  Jeff

  Shaw

  University of Nebraska at Kearney

  Vanderbilt University Medical Center

  Creighton University School of Medicine

  Country Inn & Suites by Radisson

  Creighton University

  Nashville

  TN

  Project 1: Identifying biomarkers of Helicobacter pylori-associated gastric cancer to aid in the early detection of disease.\n- Troubleshooting mass spectrometry imaging technique to distinguish unique attributes of stomach cancer.\n- Utilizing rodent (mouse and gerbil) models of H. pylori infection.\n- Determining the influence of diet on the development

  progression

  and severity of gastric cancer.\n\nProject 2: Studying signaling mechanisms that affect cellular physiology and govern H. pylori virulence.\n- Identifying novel extracellular signals that govern H. pylori virulence using next-generation sequencing (NGS) RNAseq and traditional quantitative PCR.\n- Developing innovative proteomic methods to identify the redox-active proteome of H. pylori in collaboration with core facilities.\n- Performing large-scale metabolomics study to uncover cellular alterations that occur in response to different environmental conditions and stresses.

  Postdoctoral Research Fellow

  Vanderbilt University Medical Center

  Kearney

  NE

  -Provided customer service and support in sales and by resolving customer issues and complaints in a timely and resourceful manner. \n-Documented and tracked customer requests and preferences to improve customer satisfaction. \n-Effectively communicated with management to identify customer concerns

  resolve problems

  and improve service.

  Front Desk Representative

  Country Inn & Suites by Radisson

  Kearney

  NE

  -Prepared and delivered two lectures each semester for a class of more than 100 students.

  Guest Lecturer

  Introductory Microbiology

  University of Nebraska at Kearney

  Kearney

  NE

  -Trained in research techniques covering broad areas of biology

  including ecology

  microbiology

  molecular biology

  evolution

  and bioinformatics.\n-Participated in ten weeks of research laboratory training at the University of Nebraska Medical Center as part of the NIH IDeA Networks of Biomedical Research Excellence (INBRE) program.\n-Performed research at the Kearney campus on an original project involving molecular and bioinformatic techniques to organize and analyze data concerning mycorrhizal fungi.

  Undergraduate Research Scholar

  University of Nebraska at Kearney

  Kearney

  NE

  -Taught 2-3 sections of microbiology labs consisting of 15-24 students each semester.\n-Maintained close communication with other lab instructors to ensure consistency between sections.

  Teaching Assistant

  Introductory Microbiology Laboratory

  University of Nebraska at Kearney

  Omaha

  NE

  -Utilized a diverse range of medical microbiology and molecular biology techniques

  including Next-Generation Sequencing (NGS) sample and library preparation; Sanger sequencing; quantitative PCR (qPCR); droplet digital PCR (ddPCR); protein purification

  expression

  and analysis; enzyme biochemistry and kinetic analysis; Western blot; and ELISA.\n-Developed and solved problems associated with a novel methodology for assessing bacterial fitness utilizing state-of-the-art molecular techniques.\n-Trained

  supervised

  and mentored undergraduate researchers to effectively and efficiently perform molecular and microbiology techniques in a coordinated effort.

  Research Scholar

  Ph.D.

  Creighton University

  Omaha

  NE

  -Worked with a team of instructors to design and teach an introductory microbiology course for a class of approximately 80 undergraduate nursing students.\n-Prepared and delivered 10 lectures throughout the semester.\n-Designed informal

  real-time assessments to measure student learning

  as well as evaluate and improve my teaching effectiveness throughout the course.

  Co-instructor

  Foundations in Microbiology

  Creighton University School of Medicine

  Omaha

  NE

  -Operated efficiently with the course director to organize the laboratory component of an infectious diseases course for more than 150 second year medical students.\n-Provided hands-on assistance and feedback to students performing microbiology techniques.

  Teaching Assistant

  Medical Student Infectious Diseases Laboratory

  Creighton University School of Medicine

  Kearney

  NE

  -Built relationships and established connections with the research community at conferences and meetings.\n-Established an independent research project with relevance to human health.

  Research Scholar

  M.S.

  University of Nebraska at Kearney

  American Society for Microbiology Meeting of Missouri and Missouri Valley Branches

  Vanderbilt Infection Pathogenesis Epidemiology Research (VIPER) T32 Grant

  National Institutes of Health

  First Place Presentation

  Metabolism of Infectious Diseases Conference

  Colorado State University

  Invited Talk

  National Institutes of Health

  National Institute of Allergy and Infectious Diseases

  Rocky Mountain Laboratories

  Hamilton

  MT

  Research Services Council Graduate Student Grant

  University of Nebraska

  Champion

  INBRE Meeting Limerick Competition

  Nebraska Institutional Development Award Program (IDeA) Networks of Biomedical Research Excellence (INBRE) program

  Second Place Presentation

  Midwest Student Biomedical Research Forum

  First Place Presentation

  American Society for Microbiology Meeting of Missouri and Missouri Valley Branches

  First Place Presentation

  American Society for Microbiology Meeting of Missouri and Missouri Valley Branches

  Travel Award

  Metabolism of Infectious Diseases Conference

  Colorado State University