Resume

• 7045311

  Vitronectin/Plasminogen Activator Inhibitor Interaction

  Initiated

  wrote independent research grant entitled “Vitronectin/Plasminogen Activator Inhibitor Interaction” which was funded by the American Heart Association (California Affiliate) from 1992-1994 (Grant #92-70).

  American Heart Association

  Biotechnology-Immunology-Oncology (B.I.O) Innovation Award

  In recognition of acheiving exceptional business results by demonstrating innovation and creativity for significant contributions to the development of novel intranasal device/delivery technology

  Johnson & Johnson

  Invited Speaker at Mini-Symposium on New Technologies

  Presented data used for published paper entitiled \"Cellular dielectric spectroscopy: A powerful new approach to label-free cellular analysis\"

  listed in publications section

  Society for Biomolecular Screening

  Graduate School of Health Sciences

  Albany Medical College

  Doctor of Philosophy (Ph.D.)

  Physiology and Cell Biology

  Biology

  BA

• 2017

  Community College of Vermont

  St. Alban

  VT

  Teach Anatomy & Physiology (with lab) course to allied health students.

  Adjunct Professor

  Community College of Vermont

• 2015

  Mission College

  Santa Clara

  CA

  Taught Human Physiology (with lab)

  Anatomy & Physiology (with lab) courses to allied health students.

  Adjunct Professor

  Mission College

• 2014

  Science from Scientists

  San Mateo

  CA

  Taught 4th -8th grade elementary school students STEM related science modules (includes all sciences

  Physics-Biology & Engineering); created and maintained kit modules.

  Instructor/Mentor/Kit Manager

  Science from Scientists

• 2013

  Foothill College

  Los Altos Hill

  CA

  Taught Anatomy & Physiology (with lab)

  & Microbiology (with lab) courses to allied health students.

  Adjunct Professor

  Foothill College

• 2013.

  Fremont

  CA

  Developed intranasal to brain in vivo animal model

  drug delivery platform that allows therapeutic proteins to bypass the blood brain barrier. Designed key ELISAs and DELFIAs to detect sub-nanomolar quantities of delivered proteins in rat tissues. Built and directed a team of skilled laboratory animal technicians to execute complex in vivo experiments. Managed in vivo work at all levels; wrote all IACUC protocols. Related work reference: Efflux of monoclonal antibodies from rat brain by neonatal Fc receptor

  FcRn. Brain Research 1534:13-21

  Principal Scientist

  Johnson & Johnson

• 2005

  Cañada College

  Johnson & Johnson

  Redwood City

  CA

  Taught Human Physiology course to undergraduates. Frequently successful at teaching complex biological concepts to people with no scientific background.

  Adjunct Professor

  Cañada College

  Ian Gibbons

• 2002

  Signature BioScience Inc./ MDS Sciex

  Developed cell based and enzymatic assays on state of the art microwave technology that incorporated micro and radio wave energy absorption and reflection to interrogate enzyme kinetics and endogenous receptor activation of GPCR

  1TM

  and nuclear receptors in cells

  with elucidation of underlying physiological responses

  in a label free fashion. Published paper on Cellular Dielectric Spectroscopy (see below)

  Signature BioScience Inc./ MDS Sciex

• 1998

  Gary

  Ciambrone

  ACLARA BioSciences

  Developed and patented microfluidic technology for whole cell surface enzymatic activity

  GPCR ligand binding and functional analysis. (see below US Patent # 7045311)

  ACLARA BioSciences

• 1996

  Hitachi Chemical Diagnostics

  Enhanced and improved solid phase chemiluminescent based immunoassay technology and contributed significantly to the development of a new detection system for a new quantitative immunoassay.\n-Became certified in ISO 9001 documentation.

  Hitachi Chemical Diagnostics

• 1994

  The Burnham Institute

  Optimized purification protocols for osteopontin and for investigation and elucidation of signal transduction mechanisms through chimeric integrin receptors upon binding to matrix molecules.

  The Burnham Institute

• Biotechnology

  Monoclonal Antibodies

  Physiology

  ELISA

  HPLC

  Capillary Electrophoresis

  Formulation

  Affinity Chromatography

  Biopharmaceuticals

  Protein Labeling

  Protein Assays

  Drug Delivery

  Fluorescent assays

  Invivo Animal Model Development

  Pharmacokinetics

  Protein Chemistry

  Cell Biology

  Cell based assays

  Biomarkers

  Protein Purification

  Efflux of Monoclonal Antibodies from Rat Brain by Neonatal Fc Receptor

  FcRn

  Pamela Hornby

  Yiqing Feng

  Qianqiu Li

  Lowell Johnson

  Eva Maze

  Connie Kliwinski

  Philip Cooper

  Efflux of Monoclonal Antibodies from Rat Brain by Neonatal Fc Receptor

  FcRn

  McKeown-Longo PJ

  Plasminogen activator inhibitor type-1 stabilizes vitronectin dependent adhesions in HT-1080 cells.

  McKeown-Longo PJ

  Vitronectin regulates the synthesis and localization of urokinase-type plasminogen activator inhibitor in HT-1080 cells.

  McKeown-Longo PJ

  Incubation of HT-1080 cells with polyclonal anti-plasminogen activator inhibitor antibody (aPAI-1) results in disruption of cell substratum adhesion.

  Pitchford S

  Leung GK

  McGuinness RP

  Lin DC

  Liu VF

  Cellular dielectric spectroscopy: A powerful new approach to label-free cellular analysis.

  Bell DR

  Saba TM

  Gudewicz PW

  Extravascular albumin localization in rat lung after human a-thrombin or endotoxin infusion.

  Seiffert D

  Wagner NV

  Loskutoff DJ

  Functional characterization of the somatomedin B domain of vitronectin: Stabilization of plasminogen activator inhibitor type-1 (PAI-1) activity.

  McKeown-Longo PJ

  HT-1080 fibrosarcoma cells maintain adhesion by interacting with vitronectin (VN)-plasminogen activator inhibitor type-1 (PAI-1) complexes.

  McKeown-Longo PJ

  Vitronectin mediated adhesion results in the aggregation of urokinase receptors on the surface of HT-1080 cells.