Resume

• 2015

  Home Schooling

  Guilford Technical Community College

  Evidence Based STEM teaching

  Design and Interpretation of Clinical Trials

  Johns Hopkins University via Coursera

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  What is Data Science?

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  Coursera (IBM)

  Archive Vision and Change Scholar

  American Physiological Society

  AXFF6PYU6B

  Epigenetic Control of Gene Expression

  University of Melbourne (via Coursera)

  Online Teaching Essentials

  The City University of New York

• 1994

  Columbia University

  Brooklyn College

  Winston-Salem State University

  New Mexico Highlands University

  New York City Metropolitan Area

  Lecturer (Doctoral Schedule) and Coordinator of Core Biology

  Brooklyn College

  WSSU is an HBCU mainly emphasizing teaching but moving more in a research direction. I am working on the forefront of both the teaching and research priorities. On the teaching

  I am curious in why the traditional techniques I learned by are not as effective as before

  and am one of the leaders in trying new approaches and technologies to student learning

  especially to non-biology majors. I am also

  with some of my colleagues

  designing new topical non-major courses

  such as environmental biology

  human biology and disease

  and human reproduction and development. I personally designed or co-designed two of those courses. I have also reviewed and edited a number of non-major textbooks and lab books. For research my main interest is in hormonal modulation of neurotransmitter systems. My main hormones of interest are insulin

  cortisols

  and progesterones. My research may have implications in diabetes

  food intake

  inflammation and autoimmunity

  PMDD

  anxiety

  stress

  and effects of environmental exposure to hormone mimetics. I am also screening potential drugs designed for other neurotransmitter systems for cross-effects at GABA-A receptors.

  Winston-Salem State University

  Adjunct Professor

  I taught Anatomy and Physiology I lecture and lab

  including the cat dissections

  Guilford Technical Community College

  New Mexico Highlands University

  Las Vegas

  NM

  Recruited via an NIH grant. I started the steroid work I have recently expanded and published.\nTaught biomolecules

  endocrinology

  graduate and undergraduate cell biology

  graduate genes and disease seminar; and a mixed graduate/undergraduate new course in neurobiology.

  Assistant Professor of Cell Biology

  I worked on my research project on the effects of different drugs on the shape of the GABA-A receptor.

  Columbia University

  Mount Ida College

  Newton

  MA

  In charge of Natural and Applied Sciences

  biology

  chemistry

  forensic science

  and environmental sustainability

  including all faculty

  annual reports

  assessment

  budgets

  and hiring.

  Associate Professor and Chair of Natural and Applied Sciences

  PhD

  The Integrated Program. Gave experience in microbiology

  genetics

  cancer

  and development as well as the neuroscience/hormone related work I currently do.

  Cellular

  Molecular and Biophysical Studies

  Cellular Membranes and Organelles

  Cell and Tissue Culture

  Eukaryotic Molecular Biology

  Immunology and Serology

  Biology of Cancer

  Advanced Biochemistry

  Calculus for Business

  Economics and Biology

  Cell Ultrastructure and Function

  General Physiology

  Intro to Pharmacology

  Classroom Management

  Genetics

  Advanced Cell and Developmental Biology

  General Ecology

  Cytogenetics

  Chemistry II

  Calculus II

  Education Practicum

  Immunology

  Coursera (University of Toronto)

  Bioinfomatic Methods I

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• 1992

  Rutgers University

  National Institutes of Health

  Guilford Technical Community College

  I taught Labs for General Biology

  Molecular Biology

  and Genetics.

  Rutgers University

  Marymount Manhattan College

  Greater New York City Area

  Teaching Anatomy

  Evolution

  and Drugs and Brain.

  Visiting Associate Professor of Biology

  Special pharmacology research fellow in NINDS/NIGMS working on dopamine receptor pharmacology

  desensitization

  and signaling.

  National Institutes of Health

  Society for Neuroscience

  American Association for the Advancement of Science

  Attending various meetings and workshops for major and course curriculum redesign based current research and in Vision and Change.

  AAC&U and PKAL

  Member

  American Society for Pharmacology and Experimental Therapeutics

  English

  Global Medical Discovery Article of Interest:

  For Williams DB (2011) A mutant residue in the third transmembrane region of the GABA-A alpha1 subunit causes increased agonistic neurosteroid responses. Neurochem Int. 58: 794-803. (doi:10.1016/j.neuint.2011.03.005)

  Tri Beta

  Induction as honorary member

  Research Highlighted in Target Intelligence Service

  For Williams DB (2011) A mutant residue in the third transmembrane region of the GABA-A alpha1 subunit causes increased agonistic neurosteroid responses. Neurochem Int. 58: 794-803. (doi:10.1016/j.neuint.2011.03.005)

  Faculty of 1000

  MS

  Teaching assistant for genetics

  general biology

  molecular biology

  \nMasters' research on hormone effects on GABA-A receptor ligand binding.\n

  Biology

  Coral Lansbury Award for Best Graduate Student

• 1989

  BA

  Biology

  Rahman Award as top biology student.\nAthenaeum Honor Society

• 1985

  I was co-valedictorian.

  Highland Regional High School

• JDRF

  Parent Mentor and other activities

  Cell Biology

  Scientific Writing

  Research

  Curriculum Design and Assessment

  Educational Assessment

  Classroom

  Pharmacology

  Instructional Design

  Genetics

  Teaching

  Cell Signaling

  Cell Culture

  Curriculum Development

  College Teaching

  Proposal Writing

  Teaching Writing

  Molecular Biology

  Electrophysiology

  Animal Welfare

  University Teaching

  Inhibitory effects if insulin on GABA-A currents modulated by the GABA-A alpha subunit

  Insulin

  when co-applied with GABA

  can cause an inhibition of the induced current at GABAA receptors. Main methods: This study investigated that inhibitory effect of insulin at a variety of receptor isoforms

  concentrating on α1

  α2

  and α4 containing receptors. Various isoforms were expressed in Xenopus oocytes and currents determined using two-electrode voltage clamp. Key findings: Submaximal GABA currents at all isoforms studied were inhibited by nanomolar concentrations of insulin. At α2 and α4 containing forms

  insulin could inhibit maximal GABA currents. The ability to inhibit maximal currents

  and the general potency and effects at submaximal currents paralleled the number of potential MAPK sites on the α subunits. Significance: The differences in insulin inhibition of GABA currents at different α containing GABAA receptors could be important in autocrine and paracrine control of hormone secretion in the pancreas

  and in control of reward and food intake circuits of the brain.

  Inhibitory effects if insulin on GABA-A currents modulated by the GABA-A alpha subunit

  Pregnane derived steroids have agonistic and antagonistic actions at GABAA receptors. Putative binding sites for agonistic neurosteroids are located within the transmembrane (TM) regions. A mutation within the rat α1 TM3 region

  S299C

  caused the expressed receptors to have unusual and extreme sensitivity to agonistic neurosteroids. For mutant α1S299C receptors

  with wild type β and γ subunits

  expressed in Xenopus oocytes

  steroids activated the GABAA receptors in the absence of GABA. Maximal steroid induced currents were about half of maximal GABA currents. The steroid activation was biphasic with EC50’s much lower than wild type

  in subnanomolar and nanomolar concentrations

  while the wild type had only one activation peak with near micromolar EC50. These currents could be blocked by both picrotoxin and an antagonist neurosteroid. The steroids did not seem to potentiate significantly submaximal GABA currents. The α1S299C mutation did not affect responses to the extracellularly acting partial agonist piperidine-4-sulfate. Substituted cysteine experiments indicate that this mutant can be modified by pCMBS- when the sulfhydryl reagent is added with the higher steroid concentration for activation but not the lower steroid concentration. The pCMBS- will also immediately block the high concentration steroid current. Taken together the data suggest that α1S299 is at least important in the in transduction of the steroid binding to the rest of the receptor.

  A mutant residue in the third transmembrane region of the GABA-A alpha1 subunit causes increased agonistic neurosteroid responses.

  In the CNS

  GABA and insulin seem to contribute to similar processes

  including neuronal survival; learning and reward; and energy balance and food intake. It is likely then that insulin and GABA may interact

  perhaps at the GABAA receptor. One such interaction has already been described [33]; in it a micromolar concentration of insulin causes the insertion of GABAA receptors into the cell membrane

  increasing GABA current. I have discovered another effect of insulin on GABAA currents. Using a receptor isoform α1β2γ2s that is the likely main neuronal GABAA isoform expressed recombinantly in Xenopus oocytes

  insulin inhibits GABA induced current when applied simultaneously with low concentrations of GABA. Insulin will significantly inhibit currents induced by EC30-50 concentrations of GABA by about 40%. Insulin is potent in this effect; IC50’s of insulin in a two site model were found to be about 4.5 x10-10 M. The insulin effect on the GABA dose responses looked like that of a competitive antagonist. However

  an effect of phosphorylation on the GABAA from the insulin receptor signal transduction pathway cannot yet be dismissed.

  A novel

  rapid inhibitory effect of insulin on α1β2γ2S γ-aminobutyric acid type A receptors.

  \n\n\n\n

  Letter to the Editor of Nexus Magazine

  Continuing to gather preliminary data on histamine-GABA-A receptor ligand interactions.\n

  Society for Neuroscience poster 2014

  Williams

  Daniel

  Marymount Manhattan College

  Mount Ida College