Christos S. Dimos
- Courses6
- Reviews10
- School: Johnson and Wales University
- Campus:
- Department: Science
- Email address: Join to see
- Phone: Join to see
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Location:
8 Abbott Park Pl
Providence, RI - 02903 - Dates at Johnson and Wales University: May 2012 - January 2019
- Office Hours: Join to see
Biography
Johnson and Wales University - Science
Associate Professor & Science Department Chair at Johnson & Wales University
Higher Education
Christos
Dimos
Providence, Rhode Island Area
I have always been fascinated with micro and molecular biology. As an undergraduate at Sacred Heart University, I studied the role the HS8A gene played in stress response in a soil amoeba called Dictyostelium discodeum. My Ph.D. research at The University of Rhode Island focused on discovering the function of the Cellulose Synthase-Like D (CSLDs) gene family in the moss Physcomitrella patens by performing targeted gene knockout, RNAi global silencing, and RT-qPCR studies. I also completed a comprehensive polysaccharide labeling study using various monoclonal antibodies and Carbohydrate Binding Modules (CBMs). While at the University of Rhode Island, I gained extensive teaching experience as a Teaching Assistant. I brought my passion for teaching to Johnson & Wales University, teaching General Biology-Cellular and Microbiology. I am currently developing undergraduate research projects focused on plant-pathogen interactions using the moss P. patens.
I have experience with the following experimental techniques: Northern and Southern Blots, affinity cytochemistry, florescent microscopy, morphometric analysis, transient RNAi systems, moss transformation, vector design and construction, and Reverse Transcriptase quantitative PCR.
Experience
University of Rhode Island
Teaching Assistant/Lab Coordinator
Teaching Assistant:
BIO101: Principals of Biology I Lab
BIO102: Principals of Biology II Lab
BIO321: Plant Diversity Lab
Lab Coordinator:
BIO102: Principals of Biology II Lab
BIO321: Plant Diversity LabJohnson & Wales University
Associate Professor & Science Department Chair
Classes I have taught, or I am currently teaching include:
BIO1011: General Biology- Cellular
BIO1015: General Biology Laboratory- Cellular
BIO4011: Microbiology
BIO4015: Microbiology Laboratory
BIO4070: Immunology
CHM3040: Biochemistry
PAS5200: Foundations of Medicine (Immunology & Microbiology Modules)
SCI1015: Introduction to Life Science
SCI1005: Introduction to Botany
Education
Sacred Heart University
BS
Biology, Chemistry
University of Rhode Island
Ph.D.
Biological Sciences (Plant Molecular Biology)
University of Rhode Island
Teaching Assistant/Lab Coordinator
Teaching Assistant: BIO101: Principals of Biology I Lab BIO102: Principals of Biology II Lab BIO321: Plant Diversity Lab Lab Coordinator: BIO102: Principals of Biology II Lab BIO321: Plant Diversity Lab
Publications
Introductory Science Laboratory- Writing Activities 1-3
Life Science Teaching Resource Community
Underprepared college students often have issues writing the results and discussion sections of a lab report. Here are three active learning lab-writing activities that are designed to introduce students to three different types of scientific writing skills (writing results sections for tables, writing results sections for figures, and writing discussion statements).
Introductory Science Laboratory- Writing Activities 1-3
Life Science Teaching Resource Community
Underprepared college students often have issues writing the results and discussion sections of a lab report. Here are three active learning lab-writing activities that are designed to introduce students to three different types of scientific writing skills (writing results sections for tables, writing results sections for figures, and writing discussion statements).
Scientific Literacy - Primary Research Article vs. Lay Press Secondary Article
Life Science Teaching Resource Community
This three-part assignment is designed to introduce college freshmen to the major differences in form and function between primary research articles and secondary lay press articles. The assignment supports Vison & Change core competencies and disciplinary practice six, which is the ‘ability to communicate and collaborate with other discipline’ (Brew & Smith, 2011).
Introductory Science Laboratory- Writing Activities 1-3
Life Science Teaching Resource Community
Underprepared college students often have issues writing the results and discussion sections of a lab report. Here are three active learning lab-writing activities that are designed to introduce students to three different types of scientific writing skills (writing results sections for tables, writing results sections for figures, and writing discussion statements).
Scientific Literacy - Primary Research Article vs. Lay Press Secondary Article
Life Science Teaching Resource Community
This three-part assignment is designed to introduce college freshmen to the major differences in form and function between primary research articles and secondary lay press articles. The assignment supports Vison & Change core competencies and disciplinary practice six, which is the ‘ability to communicate and collaborate with other discipline’ (Brew & Smith, 2011).
Knocking Out the Wall: Protocols for Gene Targeting in Physcomitrella patens
The Plant Cell Wall
The moss Physcomitrella patens has become established as a model for investigating plant gene function due to the feasibility of gene targeting. The chemical composition of the P. patens cell wall is similar to that of vascular plants and phylogenetic analyses of glycosyltransferase sequences from the P. patens genome have identified genes that putatively encode cell wall biosynthetic enzymes, providing a basis for investigating the evolution of cell wall polysaccharides and the enzymes that synthesize them. The protocols described in this chapter provide methods for targeted gene knockout in P. patens, from constructing vectors and maintaining cultures to transforming protoplasts and analyzing the genotypes and phenotypes of the resulting transformed lines.
Introductory Science Laboratory- Writing Activities 1-3
Life Science Teaching Resource Community
Underprepared college students often have issues writing the results and discussion sections of a lab report. Here are three active learning lab-writing activities that are designed to introduce students to three different types of scientific writing skills (writing results sections for tables, writing results sections for figures, and writing discussion statements).
Scientific Literacy - Primary Research Article vs. Lay Press Secondary Article
Life Science Teaching Resource Community
This three-part assignment is designed to introduce college freshmen to the major differences in form and function between primary research articles and secondary lay press articles. The assignment supports Vison & Change core competencies and disciplinary practice six, which is the ‘ability to communicate and collaborate with other discipline’ (Brew & Smith, 2011).
Knocking Out the Wall: Protocols for Gene Targeting in Physcomitrella patens
The Plant Cell Wall
The moss Physcomitrella patens has become established as a model for investigating plant gene function due to the feasibility of gene targeting. The chemical composition of the P. patens cell wall is similar to that of vascular plants and phylogenetic analyses of glycosyltransferase sequences from the P. patens genome have identified genes that putatively encode cell wall biosynthetic enzymes, providing a basis for investigating the evolution of cell wall polysaccharides and the enzymes that synthesize them. The protocols described in this chapter provide methods for targeted gene knockout in P. patens, from constructing vectors and maintaining cultures to transforming protoplasts and analyzing the genotypes and phenotypes of the resulting transformed lines.
Rhetorical Situations: Scientific Laboratory Writing vs. English Composition
Life Science Teaching Resource Community
This active learning assignment is designed to help freshmen science majors identify the major difference between the descriptive writing being assigned in their English compositions courses, and the descriptive writing being assigned in their introductory science laboratories.
Introductory Science Laboratory- Writing Activities 1-3
Life Science Teaching Resource Community
Underprepared college students often have issues writing the results and discussion sections of a lab report. Here are three active learning lab-writing activities that are designed to introduce students to three different types of scientific writing skills (writing results sections for tables, writing results sections for figures, and writing discussion statements).
Scientific Literacy - Primary Research Article vs. Lay Press Secondary Article
Life Science Teaching Resource Community
This three-part assignment is designed to introduce college freshmen to the major differences in form and function between primary research articles and secondary lay press articles. The assignment supports Vison & Change core competencies and disciplinary practice six, which is the ‘ability to communicate and collaborate with other discipline’ (Brew & Smith, 2011).
Knocking Out the Wall: Protocols for Gene Targeting in Physcomitrella patens
The Plant Cell Wall
The moss Physcomitrella patens has become established as a model for investigating plant gene function due to the feasibility of gene targeting. The chemical composition of the P. patens cell wall is similar to that of vascular plants and phylogenetic analyses of glycosyltransferase sequences from the P. patens genome have identified genes that putatively encode cell wall biosynthetic enzymes, providing a basis for investigating the evolution of cell wall polysaccharides and the enzymes that synthesize them. The protocols described in this chapter provide methods for targeted gene knockout in P. patens, from constructing vectors and maintaining cultures to transforming protoplasts and analyzing the genotypes and phenotypes of the resulting transformed lines.
Rhetorical Situations: Scientific Laboratory Writing vs. English Composition
Life Science Teaching Resource Community
This active learning assignment is designed to help freshmen science majors identify the major difference between the descriptive writing being assigned in their English compositions courses, and the descriptive writing being assigned in their introductory science laboratories.
Immuno and Affinity Cytochemical Analysis of Cell Wall Composition in the Moss Physcomitrella patens
Frontiers in Plant Science
In contrast to homeohydric vascular plants, mosses employ a poikilohydric strategy for surviving in the dry aerial environment. A detailed understanding of the structure, composition, and development of moss cell walls can contribute to our understanding of not only the evolution of overall cell wall complexity, but also the differences that have evolved in response to selection for different survival strategies. The model moss species Physcomitrella patens has a predominantly haploid lifecycle consisting of protonemal filaments that regenerate from protoplasts and enlarge by tip growth, and leafy gametophores composed of cells that enlarge by diffuse growth and differentiate into several different types. Advantages for genetic studies include methods for efficient targeted gene modification and extensive genomic resources. Immuno and affinity cytochemical labeling were used to examine the distribution of polysaccharides and proteins in regenerated protoplasts, protonemal filaments, rhizoids, and sectioned gametophores of P. patens. The cell wall composition of regenerated protoplasts was also characterized by flow cytometry. Crystalline cellulose was abundant in the cell walls of regenerating protoplasts and protonemal cells that developed on media of high osmolarity, whereas homogalactuonan was detected in the walls of protonemal cells that developed on low osmolarity media and not in regenerating protoplasts. Mannan was the major hemicellulose detected in all tissues tested. Arabinogalactan proteins were detected in different cell types by different probes, consistent with structural heterogneity. The results reveal developmental and cell type specific differences in cell wall composition and provide a basis for analyzing cell wall phenotypes in knockout mutants.
