The Ohio State University

Assistant Professor

Molecular biology research, focusing on the interface between signal transduction and epigenetics. Special interest in mechanisms of genomic imprinting.

University of Colorado Denver

Assistant Professor

Molecular biology research, focusing on the interface between signal transduction, cell biology and epigenetics. Main focus is understanding how Gsk-3 activity is involved in embryonic stem cell pluripotency.

University of Colorado Denver

Associate Professor

Christopher worked at University of Colorado Denver as a Associate Professor

Magainin Pharmaceuticals

Research assistant

Lab assistant in peptide chemistry.

Targeted disruption of GSK-3α affects sperm motility resulting in male infertility

Biology of Reproduction

Targeted disruption of GSK-3α affects sperm motility resulting in male infertility

Biology of Reproduction

Live-cell single-molecule dynamics of PcG proteins imposed by the DIPG H3.3K27M mutation

Nature Communications

Targeted disruption of GSK-3α affects sperm motility resulting in male infertility

Biology of Reproduction

Live-cell single-molecule dynamics of PcG proteins imposed by the DIPG H3.3K27M mutation

Nature Communications

Glycogen Synthase Kinase-3α Promotes Fatty Acid Uptake and Lipotoxic Cardiomyopathy

Cell Metabolism

Targeted disruption of GSK-3α affects sperm motility resulting in male infertility

Biology of Reproduction

Live-cell single-molecule dynamics of PcG proteins imposed by the DIPG H3.3K27M mutation

Nature Communications

Glycogen Synthase Kinase-3α Promotes Fatty Acid Uptake and Lipotoxic Cardiomyopathy

Cell Metabolism

Glycogen synthase kinase-3 (GSK3) activity regulates mRNA methylation in mouse embryonic stem cells

Journal of Biological Chemistry

Targeted disruption of GSK-3α affects sperm motility resulting in male infertility

Biology of Reproduction

Live-cell single-molecule dynamics of PcG proteins imposed by the DIPG H3.3K27M mutation

Nature Communications

Glycogen Synthase Kinase-3α Promotes Fatty Acid Uptake and Lipotoxic Cardiomyopathy

Cell Metabolism

Glycogen synthase kinase-3 (GSK3) activity regulates mRNA methylation in mouse embryonic stem cells

Journal of Biological Chemistry

A Simple and Efficient Method for Transfecting Mouse Embryonic Stem Cells Using Polyethylenimine

Experimental Cell Research

Targeted disruption of GSK-3α affects sperm motility resulting in male infertility

Biology of Reproduction

Live-cell single-molecule dynamics of PcG proteins imposed by the DIPG H3.3K27M mutation

Nature Communications

Glycogen Synthase Kinase-3α Promotes Fatty Acid Uptake and Lipotoxic Cardiomyopathy

Cell Metabolism

Glycogen synthase kinase-3 (GSK3) activity regulates mRNA methylation in mouse embryonic stem cells

Journal of Biological Chemistry

A Simple and Efficient Method for Transfecting Mouse Embryonic Stem Cells Using Polyethylenimine

Experimental Cell Research

Immobilization of PRC1 Complex at Mitotic Chromosomes by Cbx2 in a PRC2-Independent Mechanism

Molecular Biology of the Cell

Targeted disruption of GSK-3α affects sperm motility resulting in male infertility

Biology of Reproduction

Live-cell single-molecule dynamics of PcG proteins imposed by the DIPG H3.3K27M mutation

Nature Communications

Glycogen Synthase Kinase-3α Promotes Fatty Acid Uptake and Lipotoxic Cardiomyopathy

Cell Metabolism

Glycogen synthase kinase-3 (GSK3) activity regulates mRNA methylation in mouse embryonic stem cells

Journal of Biological Chemistry

A Simple and Efficient Method for Transfecting Mouse Embryonic Stem Cells Using Polyethylenimine

Experimental Cell Research

Immobilization of PRC1 Complex at Mitotic Chromosomes by Cbx2 in a PRC2-Independent Mechanism

Molecular Biology of the Cell

Glycogen Synthase Kinase-3 (Gsk-3) Plays A Fundamental Role In Maintaining DNA Methylation At Imprinted Loci In Mouse Embryonic Stem Cells

Molecular Biology of the Cell

Glycogen synthase kinase-3 (Gsk-3) is a key regulator of multiple signal transduction pathways. Recently, we described a novel role for Gsk-3 in the regulation of DNA methylation at imprinted loci in mouse embryonic stem cells (ESCs), suggesting that epigenetic changes regulated by Gsk-3 are likely an unrecognized facet of Gsk-3 signaling. Here, we extend our initial observation to the entire mouse genome by enriching for methylated DNA with the MethylMiner kit and performing next-generation sequencing (MBD-Seq) in wild-type and Gsk-3α-/-;Gsk-3β-/- ESCs. Consistent with our previous data, we found that 77% of known imprinted loci have reduced DNA methylation in Gsk-3-deficient ESCs. More specifically, we unambiguously identified changes in DNA methylation within regions that have been confirmed to function as imprinting control regions (ICRs). In many cases, the reduced DNA methylation at imprinted loci in Gsk-3α-/-;Gsk-3β-/- ESCs was accompanied by changes in gene expression as well. Furthermore, many of the Gsk-3-dependent differentially methylated regions (DMRs) are identical to the DMRs recently identified in uniparental ESCs. Our data demonstrate the importance of Gsk-3 activity in the maintenance of DNA methylation at a majority of the imprinted loci in ESCs, and emphasizes the importance for Gsk-3-mediated signal transduction on the epigenome.

Targeted disruption of GSK-3α affects sperm motility resulting in male infertility

Biology of Reproduction

Live-cell single-molecule dynamics of PcG proteins imposed by the DIPG H3.3K27M mutation

Nature Communications

Glycogen Synthase Kinase-3α Promotes Fatty Acid Uptake and Lipotoxic Cardiomyopathy

Cell Metabolism

Glycogen synthase kinase-3 (GSK3) activity regulates mRNA methylation in mouse embryonic stem cells

Journal of Biological Chemistry

A Simple and Efficient Method for Transfecting Mouse Embryonic Stem Cells Using Polyethylenimine

Experimental Cell Research

Immobilization of PRC1 Complex at Mitotic Chromosomes by Cbx2 in a PRC2-Independent Mechanism

Molecular Biology of the Cell

Glycogen Synthase Kinase-3 (Gsk-3) Plays A Fundamental Role In Maintaining DNA Methylation At Imprinted Loci In Mouse Embryonic Stem Cells

Molecular Biology of the Cell

Glycogen synthase kinase-3 (Gsk-3) is a key regulator of multiple signal transduction pathways. Recently, we described a novel role for Gsk-3 in the regulation of DNA methylation at imprinted loci in mouse embryonic stem cells (ESCs), suggesting that epigenetic changes regulated by Gsk-3 are likely an unrecognized facet of Gsk-3 signaling. Here, we extend our initial observation to the entire mouse genome by enriching for methylated DNA with the MethylMiner kit and performing next-generation sequencing (MBD-Seq) in wild-type and Gsk-3α-/-;Gsk-3β-/- ESCs. Consistent with our previous data, we found that 77% of known imprinted loci have reduced DNA methylation in Gsk-3-deficient ESCs. More specifically, we unambiguously identified changes in DNA methylation within regions that have been confirmed to function as imprinting control regions (ICRs). In many cases, the reduced DNA methylation at imprinted loci in Gsk-3α-/-;Gsk-3β-/- ESCs was accompanied by changes in gene expression as well. Furthermore, many of the Gsk-3-dependent differentially methylated regions (DMRs) are identical to the DMRs recently identified in uniparental ESCs. Our data demonstrate the importance of Gsk-3 activity in the maintenance of DNA methylation at a majority of the imprinted loci in ESCs, and emphasizes the importance for Gsk-3-mediated signal transduction on the epigenome.

Isoform-Specific Requirement for GSK-3α in Sperm for Male Fertility

Biology of Reproduction

Targeted disruption of GSK-3α affects sperm motility resulting in male infertility

Biology of Reproduction

Live-cell single-molecule dynamics of PcG proteins imposed by the DIPG H3.3K27M mutation

Nature Communications

Glycogen Synthase Kinase-3α Promotes Fatty Acid Uptake and Lipotoxic Cardiomyopathy

Cell Metabolism

Glycogen synthase kinase-3 (GSK3) activity regulates mRNA methylation in mouse embryonic stem cells

Journal of Biological Chemistry

A Simple and Efficient Method for Transfecting Mouse Embryonic Stem Cells Using Polyethylenimine

Experimental Cell Research

Immobilization of PRC1 Complex at Mitotic Chromosomes by Cbx2 in a PRC2-Independent Mechanism

Molecular Biology of the Cell

Glycogen Synthase Kinase-3 (Gsk-3) Plays A Fundamental Role In Maintaining DNA Methylation At Imprinted Loci In Mouse Embryonic Stem Cells

Molecular Biology of the Cell

Glycogen synthase kinase-3 (Gsk-3) is a key regulator of multiple signal transduction pathways. Recently, we described a novel role for Gsk-3 in the regulation of DNA methylation at imprinted loci in mouse embryonic stem cells (ESCs), suggesting that epigenetic changes regulated by Gsk-3 are likely an unrecognized facet of Gsk-3 signaling. Here, we extend our initial observation to the entire mouse genome by enriching for methylated DNA with the MethylMiner kit and performing next-generation sequencing (MBD-Seq) in wild-type and Gsk-3α-/-;Gsk-3β-/- ESCs. Consistent with our previous data, we found that 77% of known imprinted loci have reduced DNA methylation in Gsk-3-deficient ESCs. More specifically, we unambiguously identified changes in DNA methylation within regions that have been confirmed to function as imprinting control regions (ICRs). In many cases, the reduced DNA methylation at imprinted loci in Gsk-3α-/-;Gsk-3β-/- ESCs was accompanied by changes in gene expression as well. Furthermore, many of the Gsk-3-dependent differentially methylated regions (DMRs) are identical to the DMRs recently identified in uniparental ESCs. Our data demonstrate the importance of Gsk-3 activity in the maintenance of DNA methylation at a majority of the imprinted loci in ESCs, and emphasizes the importance for Gsk-3-mediated signal transduction on the epigenome.

Isoform-Specific Requirement for GSK-3α in Sperm for Male Fertility

Biology of Reproduction

Glycogen Synthase Kinase 3 Controls Migration of the Cranial Neural Crest Lineage in Mouse and Xenopus

Nature Communications

Targeted disruption of GSK-3α affects sperm motility resulting in male infertility

Biology of Reproduction

Live-cell single-molecule dynamics of PcG proteins imposed by the DIPG H3.3K27M mutation

Nature Communications

Glycogen Synthase Kinase-3α Promotes Fatty Acid Uptake and Lipotoxic Cardiomyopathy

Cell Metabolism

Glycogen synthase kinase-3 (GSK3) activity regulates mRNA methylation in mouse embryonic stem cells

Journal of Biological Chemistry

A Simple and Efficient Method for Transfecting Mouse Embryonic Stem Cells Using Polyethylenimine

Experimental Cell Research

Immobilization of PRC1 Complex at Mitotic Chromosomes by Cbx2 in a PRC2-Independent Mechanism

Molecular Biology of the Cell

Glycogen Synthase Kinase-3 (Gsk-3) Plays A Fundamental Role In Maintaining DNA Methylation At Imprinted Loci In Mouse Embryonic Stem Cells

Molecular Biology of the Cell

Glycogen synthase kinase-3 (Gsk-3) is a key regulator of multiple signal transduction pathways. Recently, we described a novel role for Gsk-3 in the regulation of DNA methylation at imprinted loci in mouse embryonic stem cells (ESCs), suggesting that epigenetic changes regulated by Gsk-3 are likely an unrecognized facet of Gsk-3 signaling. Here, we extend our initial observation to the entire mouse genome by enriching for methylated DNA with the MethylMiner kit and performing next-generation sequencing (MBD-Seq) in wild-type and Gsk-3α-/-;Gsk-3β-/- ESCs. Consistent with our previous data, we found that 77% of known imprinted loci have reduced DNA methylation in Gsk-3-deficient ESCs. More specifically, we unambiguously identified changes in DNA methylation within regions that have been confirmed to function as imprinting control regions (ICRs). In many cases, the reduced DNA methylation at imprinted loci in Gsk-3α-/-;Gsk-3β-/- ESCs was accompanied by changes in gene expression as well. Furthermore, many of the Gsk-3-dependent differentially methylated regions (DMRs) are identical to the DMRs recently identified in uniparental ESCs. Our data demonstrate the importance of Gsk-3 activity in the maintenance of DNA methylation at a majority of the imprinted loci in ESCs, and emphasizes the importance for Gsk-3-mediated signal transduction on the epigenome.

Isoform-Specific Requirement for GSK-3α in Sperm for Male Fertility

Biology of Reproduction

Glycogen Synthase Kinase 3 Controls Migration of the Cranial Neural Crest Lineage in Mouse and Xenopus

Nature Communications

Gene expression profiling in mouse embryonic stem cells reveals glycogen synthase kinase-3-dependent targets of phosphatidylinositol 3-kinase and Wnt/ß-catenin signaling pathways

Frontiers in Endocrinology