North Central Texas College - Biology
Ph.D.
Texas A&M University Regents Fellowship 2007-2008\nAmerican Society for Microbiology Student Travel Grant 2011\nPlant Pathology and Microbiology Student Travel Grant\t2011\nVolunteer Judge at the 2011 Robertson County
Texas Science Fair
Plant Pathology and Microbiology
Officer in MANRRS (Minorities in Agriculture
Natural Resources
and Related Sciences) 2008-2010\nPlant Pathology Graduate Student Club 2007-2011\nAmerican Society for Microbiology Student Member
Texas A&M University
BS
Recipient of TWU Chancellors Student Research Scholars Award 2006\nTWU Biology Department Scholarship 2006\nFirst place in the Genomics Education Poster Scholarship program
a nationwide competition sponsored by LI-COR Biosciences Corporation 2006
Biology
Officer in Kappa Epsilon Mu Chemistry Club 2005-2006\nBeta Beta Beta Biological Society
Texas Woman's University
Botany
Molecular Biology
Bioinformatics
DNA extraction
Bacterial Transformation
Plant Pathology
Microbial Ecology
Bacterial Culturing
Biofuels
DNA sequencing
Microscopy
Soil Microbiology
Biology
Higher Education
Bacterial Identification
Sequence Analysis
Environmental Microbiology
Industrial Microbiology
Microbiology
University Teaching
Comparison of three screening methods to select mixed-microbial inoculum for mixed-acid fermentations
Using a mixed culture of microorganisms
the carboxylate platform converts biomass into hydrocarbons and chemicals. To develop a method that identifies the highest performing inoculum for carboxylate fermentations
five bacterial communities were screened and ranked by three fermentation performance tests: (1) 30-day batch screen
(2) 28-day continuum particle distribution model (CPDM)
and (3) 5-month continuous countercurrent fermentation trains. To screen numerous inocula sources
these tests were used sequentially in an aseptic environment. For the batch-fermentation screen
Inoculum 1 achieved the highest conversion. For the CPDM evaluation
the operating map for Inoculum 1 had the highest performance. For the continuous countercurrent fermentation
the train resulting from Inoculum 1 was among the best performers. This study suggests that the three screens are a useful and predictive method for choosing optimal inocula sources. The bacterial community with optimal performance in these three screens could be considered for use in commercial-scale fermentations.
Comparison of three screening methods to select mixed-microbial inoculum for mixed-acid fermentations
To test the hypothesis that microbial communities from saline and thermal sediment environments are pre-adapted to exhibit superior fermentation performances
501 saline and thermal samples were collected from a wide geographic range. Each sediment sample was screened as inoculum in a 30-day batch fermentation. Using multivariate statistics
the capacity of each community was assessed to determine its ability to degrade a cellulosic substrate and produce carboxylic acids in the context of the inoculum sediment chemistry. Conductance of soils was positively associated with production of particular acids
but negatively associated with conversion efficiency. In situ sediment temperature and conversion efficiency were consistently positively related. Because inoculum characteristics influence carboxylate platform productivity
optimization of the inoculum is an important and realistic goal.
Evaluating the performance of carboxylate platform fermentations across diverse inocula originating as sediments from extreme environments
Naturally occurring microbial communities from high-salt and/or high-temperature environments were collected from sites across the United States and Puerto Rico and screened for their efficacy in the MixAlco™ biofuel production platform. The MixAlco™ process
based on the carboxylate platform
is a sustainable and economically viable platform for converting lignocellulosic biomass to biofuels. Using a mixed culture of anaerobic organisms
lignocellulosic biomass is fermented into carboxylic acids
which are neutralized to their corresponding carboxylate salts. These salts can then be converted into a wide variety of chemical products and fuels (alcohols
gasoline
diesel
jet fuel). The central hypothesis is that microbial communities from relatively extreme environments
having evolved to withstand selection pressures similar to the conditions in the carboxylate platform
will exhibit high rates of biomass conversion. A total of 559 soil communities was screened as inocula in established laboratory-scale fermentations. We used pyrotag sequencing of 16S rRNA genes to characterize the bacterial components of the best-performing microbial communities. The best performing communities converted up to 3 times more biomass to acids than a standard marine community inoculum. The community analyses have allowed us to determine the extent to which the same functional types are favored during fermentation
at both laboratory and demonstration plant scales. The wealth of data provided by current sequencing technologies allowed us to question whether communities with high process performances tend to achieve that performance with similar community structures.
Hammett
Texas Woman's University
North Central Texas College
Texas Woman's University
Texas A&M University
Tarrant County College
University of Pennsylvania
Flower Mound
Texas
Microbiology Instructor
North Central Texas College
Denton
Texas
Department of Biology faculty position responsible for preparation
set-up and supervision of biology laboratory courses including microbiology
immunology
botany
ecology
environmental science
and general science.
Laboratory Instructor
Texas Woman's University
Denton
TX
Department of Biology faculty position responsible for preparation
set-up and supervision of biology laboratory courses including microbiology
immunology
botany
ecology
environmental science
and general science.
Assistant Clinical Professor
Texas Woman's University
Denton
Texas
Nutrition and Food Sciences Adjunct Faculty Food Microbiology
Texas Woman's University
Denton
Texas
Adjunct Faculty Microbiology
Texas Woman's University
Denton
Texas
Research with Tina L. Gumienny
Ph.D.
in cell signaling using C. elegans
Research Assistant
Texas Woman's University
Philadelphia
Pennsylvania
Participant in the 2006 Summer Undergraduate Internship with Dr. Michael Sebert at the Children’s Hospital of Philadelphia investigating protease regulation of pneumococcal competence.
Internship Program in Bio-medical Science
University of Pennsylvania
Denton
Texas
Undergraduate research with Dr. Sarah McIntire investigating plasmids and their role in the pathogenicity of Helicobacter pylori.
Undergraduate Research
Texas Woman's University
Denton
Texas
Nursing Microbiology and Biology Laboratory Student Teaching Assistant. Instruction of laboratory principles and techniques to students.
Course Assistant
Texas Woman's University
Denton
Texas
Undergraduate research with Dr. Camelia Maier investigating bio-mineralization in dioecious Maclura pomifera (Osage-Orange) trees.
Undergraduate Research
Texas Woman's University
College Station
Texas
Analysis of microbial communities from extreme environments
and obtaining and screening naturally occurring microbial communities in a bio-fuel platform (MixAlco™) by comparing phylogenetic diversity between the sediment microbial communities to those same communities after the screen’s selective pressure.
Graduate Research
Texas A&M University
Denton
Texas
Undergraduate Research with Dr. Sarah McIntire and Dr. Camelia Maier investigating microbial communities associated with Dionaea muscipula (Venus Flytrap) traps.
Undergraduate Research
Texas Woman's University
Fort Worth
Texas
Microbiology Instructor
Tarrant County College
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