Resume

• 2005

  Master of Science (MS)

  Masters Of Applied Mathematics and Computational Biology(Bioinformatics) certificate

  Applied Mathematics computational biology emphasis

  University of Colorado at Denver

  SharePoint 2010 Getting Started

  DA2141

  lynda.com

  Lean Six Sigma Green Belt (ICGB)

  Go-Lean-Six Training & Consulting

  Project Management

  UC San Diego Extension

  lynda.com

  Business Writing Fundamentals

  2CD75D

• 1999

  Bachelor of Applied Science (B.A.Sc.)

  Computer Science

  Metropolitan State University of Denver

• Java

  Matlab

  Genomics

  Higher Education

  Perl

  Computational Biology

  Linux

  Bioinformatics

  Research

  Teaching

  R

  Mathematics

  Databases

  HTML

  Programming

  Data Analysis

  French

  Data Mining

  Statistics

  C++

  A comprehensive analysis of endocrine disruption in hornyhead turbot (Pleuronichthys verticalis) at five coastal sites in Southern California

  1.\tBaker ME Ribecco C Vidal-Dorsch D Sprague LJ Angert M Lekmine N Ludka C Martella A Ricciardelli A

  Current environmental monitoring programs focus primarily on legacy contaminants identified in the past as priority pollutants. Many new chemicals including so-called contaminants of emerging concern (CECs) are being widely dispersed into the environment and the development of sensitive tools that can screen endocrine responses and monitor the harmful effects of pollutants has become a priority. We developed a focused quantitative polymerase chain reaction (q-PCR) panel containing key endocrine gene targets for investigation of the effects of contaminants on transcriptional patterns in the demersal marine flatfish

  hornyhead turbot (Pleuronichthys verticalis). An additional objective was to develop this tool so that it had broad applicability for monitoring endocrine disruption in other sentinel species

  including California flatfish. This endocrine Q-PCR panel was validated by studying transcriptional perturbations in two important sentinel species

  hornyhead turbot and\nEnglish sole (Parophrys vetulus)

  both sampled from sewage outfall and reference coastal waters in Southern California. In addition

  the cross species utility of the endocrine Q-PCR panel was examined by its application to the study of gene expression changes in laboratory male zebrafish (Danio rerio) following exposure to 17-b estradiol and 4-nonylphenol.

  A comprehensive analysis of endocrine disruption in hornyhead turbot (Pleuronichthys verticalis) at five coastal sites in Southern California

  3.\tMatsui H Stenbit A Lekmine N Šášik R Valafar F Conrad D and Hardiman G.

  Application of multi-species endocrine Q-PCR Panel to monitor the effects of pollution in southern California flatfish

  2.\tMichael E. Baker L. James Sprague Cataldo Ribecco Barbara Ruggeri Narimene Lekmine Colleen Ludka Iva

  Abstract\n\nSentinel fish hornyhead turbot (Pleuronichthys verticalis) captured near wastewater outfalls are used for monitoring exposure to industrial and agricultural chemicals of ~ 20 million people living in coastal Southern California. Although analyses of hormones in blood and organ morphology and histology are useful for assessing contaminant exposure

  there is a need for quantitative and sensitive molecular measurements

  since contaminants of emerging concern are known to produce subtle effects. We developed a second generation multi-species microarray with expanded content and sensitivity to investigate endocrine disruption in turbot captured near wastewater outfalls in San Diego

  Orange County and Los Angeles California. Analysis of expression of genes involved in hormone [e.g.

  estrogen

  androgen

  thyroid] responses and xenobiotic metabolism in turbot livers was correlated with a series of phenotypic end points. Molecular analyses of turbot livers uncovered altered expression of vitellogenin and zona pellucida protein

  indicating exposure to one or more estrogenic chemicals

  as well as

  alterations in cytochrome P450 (CYP) 1A

  CYP3A and glutathione S-transferase-α indicating induction of the detoxification response. Molecular responses indicative of exposure to endocrine disruptors were observed in field-caught hornyhead turbot captured in Southern California demonstrating the utility of molecular methods for monitoring environmental chemicals in wastewater outfalls. Moreover

  this approach can be adapted to monitor other sites for contaminants of emerging concern in other fish species for which there are few available gene sequences.

  Application of multi-species endocrine Q-PCR Panel to monitor the effects of pollution in southern California flatfish

  Narimene

  Lekmine

  San Diego Community College District

  Community College of Denver

  General Atomics Aeronautical Systems

  New School of Architechture and Design

  Metropolitan State College of Denver

  University of California

  San Diego/Clinical and Translational Research Institute

  University of California

  San Diego

  Clinical and Translational Research Institute

  General Atomics Electromagnetic Systems

  UCSD Medical Center/Biogem

  San Diego

  California

  Math Instructor

  San Diego Community College District

  Community College of Denver

  General Atomics Electromagnetic Systems

  Greater San Diego Area

  Senior Systems Administrator-SharePoint Developer

  La Jolla

  Programmer Analyst/ Webmaster

  University of California

  San Diego/Clinical and Translational Research Institute

  San Diego

  University of California

  San Diego

  Clinical and Translational Research Institute

  Computer Science Instructor

  Metropolitan State College of Denver

  UCSD Medical Center/Biogem

  San Diego

  California

  Programmer Analyst

  San Diego

  California

  Programmer Analyst

  General Atomics Aeronautical Systems

  Poway

  California

  Project Manager

  General Atomics Aeronautical Systems

  San Diego

  California

  Instructor

  New School of Architechture and Design

  French

  Arabic

  English